猪GV期与MⅡ期卵母细胞的超微结构特征  

Ultra-structure of Porcine GV-and MⅡ-oocytes

作　　者：武彩红[1] 戴建军[1] 王会珍[1] 葛立军[1] 卢庆[1] 芮荣[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《江苏农业学报》2007年第4期312-316,共5页Jiangsu Journal of Agricultural Sciences

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2003)第14-1号];国家自然科学基金项目(30270958)

摘　　要：采用扫描电镜(SEM)和透射电镜(TEM)观察并比较猪未成熟(GV期)与体外成熟(MⅡ期)卵母细胞的超微结构特征。结果发现:①猪GV期卵母细胞外紧密包被着卵丘细胞,卵丘细胞突起伸入透明带内,透明带呈不规则蜂窝状;卵母细胞微绒毛相对较长、数量较多,并穿入透明带内;卵母细胞呈充满状态,卵周隙不明显;胞质内高尔基复合体发达,线粒体成簇分布于脂滴附近;脂滴表现为均质和不均质两种形式,大多以均质脂滴形式存在,脂滴周围有不连续的滑面内质网。②MⅡ期卵母细胞周围,卵丘细胞结构松散、扩展;卵母细胞微绒毛数量减少、变短、变粗,并从透明带内撤回;透明带上峰窝状小孔增多,小梁突起更为明显,卵周隙明显;线粒体仍成簇伴随脂滴分布,脂滴以不均质型为主,呈明显的絮状结构,脂滴外有不连续的滑面内质网;皮质颗粒以单层排列于质膜下。猪卵母细胞成熟前后会出现一些规律性变化,具有不同的超微结构特征。The ultra-structure of both porcine immature （ GV stage） and mature in vitro （ Metaphase Ⅱ , MⅡ ） oocytes were observed by scanning electron microscopy （ SEM ） and transmission electron microscopy （ TEM ）. The cumulus ceils enclosed each GV-oocyte compactly and their protuberances stretched into zona pellucida of the oocyte. The zona pellucida appeared a mesh-like structure with numerous fenestrations together with an uneven surface with a microtrabecular appearance. Long and abundant microvilli on the oolemma surface extended into the zona pellucida. The perivitelline space between GV-oocytes was not discernable. Inside the cytoplasm of GV-oocytes ample Golgi complexes and the mitochondria with a few clear ridges distributed next to lipid drops in clusters were observeg. Homogeneous droplets dominated the lipid droplets which were surrounded by smooth endoplasmic reticulum. All around MⅡ -oocytes ,the cumulus ceils became expended. The microvilli looked tubby and withdrew from zona pellucida. The microtrabecular on the surface of zona pellucida and the perivitelline space between MⅡ-oocytes could be observed distinctly. The inhomogeneous lipid droplets presented dominantly in global shape with more floccules. Clusters of mitochondria lied near the lipid droplets . The cortical granules were arranged beneath the oolemma membrane. The results indicated that before and after maturation, porcine oocytes did behave some regular alterations in ultra-structure.

关 键 词：猪 卵母细胞 体外成熟 超微结构 

分 类 号：S828.1[农业科学—畜牧学]