Biocompatibility of Compounds of Extracellular Matrix and Thermally Reversible Hydrogel  

作　　者：姚颐 杨嗣星 

机构地区：[1]Department of Urology,Renmin Hospital of Wuhan University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2007年第3期439-442,共4页武汉理工大学学报（材料科学英文版）

基　　金：the Natural Science Foundation of Hubei Province (No.2003ABA187);Hubei Education Bureau (No.2003X123)

摘　　要：A new scaffold material composed of extracellular matrix （ECM） and thermal sensitive hydrogel （HG）, and evaluated its biocompatibility were investigated. We cultured bladder smooth muscle cells with this compound material, and then observed with phase contrast microscopy and scanning electron microscope （SEM） to assess the cell growth and morphology. The cell adhesion and proliferation were detected with MTT assay and cell count. Results show the ECM/HG compounds appeared as a net-like and red-stained construction with enough meshes and without any cellular fragments. 6 h after implantation, cells were observed adhere on the compounds and extend spurious along the fibers 12 h later. Under SEM even some ECM was observed to be secreted. MTT assay shows there was obvious statistic difference among 3 groups （P〈0.05）. ECM/HG compound materials show a good biocompatibility, which confirms that it would be an ideal tissue engineering scaffolds.A new scaffold material composed of extracellular matrix （ECM） and thermal sensitive hydrogel （HG）, and evaluated its biocompatibility were investigated. We cultured bladder smooth muscle cells with this compound material, and then observed with phase contrast microscopy and scanning electron microscope （SEM） to assess the cell growth and morphology. The cell adhesion and proliferation were detected with MTT assay and cell count. Results show the ECM/HG compounds appeared as a net-like and red-stained construction with enough meshes and without any cellular fragments. 6 h after implantation, cells were observed adhere on the compounds and extend spurious along the fibers 12 h later. Under SEM even some ECM was observed to be secreted. MTT assay shows there was obvious statistic difference among 3 groups （P〈0.05）. ECM/HG compound materials show a good biocompatibility, which confirms that it would be an ideal tissue engineering scaffolds.

关 键 词：extracellular matrix HYDROGEL compounds materials BIOCOMPATIBILITY 

分 类 号：R318.08[医药卫生—生物医学工程]