马精液保存稀释液筛选及保存效果观察  

Stallion Semen Preservation Selection of Extenders and Effection Observation

作　　者：耿娟[1] 王慧伟[1] 陈静波[1] 谭小海 

机构地区：[1]新疆畜牧科学院 [2]新疆伊犁州昭苏马场,新疆伊犁835600

出　　处：《草食家畜》2007年第3期27-30,共4页Grass-Feeding Livestock

基　　金：新疆维吾尔自治区科技厅国际合作处项目。

摘　　要：为了提高优秀种公马的利用率,快速扩大优秀马群,在马的非繁殖季节进行常温、低温和超低温马精液保存稀释液的筛选及保存效果观察,精液采自新疆伊犁昭苏马场的4匹种公马。实验结果表明:(1)常温保存时,2.5%甘油液和7%葡萄糖液精子活率显著高于5%甘油液和0.9%NaCl液(P<0.05),存活时间极显著高于5%甘油液和0.9%NaCl液(P<0.01);(2)低温保存时,10%奶粉液和5%卵黄液精子活率显著高于0.8%卵黄液(P<0.05),极显著高于7%葡萄糖液(P<0.01),存活时间较0.8%卵黄液和7%葡萄糖液差异极显著(P<0.01);(3)冷冻保存时,11%乳糖液、10%蔗糖液和4%果糖液颗粒冻精活率均极显著高于0.1%EDTA液、3%葡萄糖液和1.25%Tris液(P<0.01);细管冻精活率显著高于3%葡萄糖液和1.25%Tris液(P<0.05),极显著高于0.1%EDTA液(P<0.01),且同一种稀释液的颗粒冻精和细管冻精的冻后活率和复苏率差异显著(P<0.05)。10%蔗糖液和4%果糖液冻后精子顶体完整率极显著高于11%乳糖液(P<0.01),畸形率显著高于11%乳糖液(P<0.05),颗粒和细管冻精差异不显著(P>0.05)。The stallion semen preservation extenders and dosage forms in different temperatures were selected in this experiment. The results showed that： （1） The sperm motility in 2.5%Gly extender, 7%Glu extender were higher significantly than that in 5% Gly extender, 0.9% Nacl extender（P〈0.05）, and the survival time was extreme significant（P〈0.01） in room temperature,. （2）In low- temperature, the sperm motility in 10%Milk powder extender, 5% Egg yolk extender were higher significantly than that in 0.8% Egg yolk extender（P〈0.05） and extreme significantly than that in 7%Glu extender（P〈0.01）, and the survival time was longer extreme significant than that in 0.8%Egg yolk extender, 7%Glu extender（P〈0.01）. （3）In cryopreservation, the pellet frozen semen motility in 11% Lac extender, 10%Suc extender, 4%Fructose extender were extreme significantly than that in 0.1%EDTA extender, 3%Glu extender, 1.25%Tris extender（P〈0.01）. The straw semen motility were higher significantly than that in 3% Glu extender, 1.25%Tris extender（P〈0.05） and 0. I%EDTA extender extreme significantly（P〈0.01）. Compared with the straw semen, the frozen-thawing motility and recovery of the pellet semen were significantly different（P〈0.05）. Acorosomal intrgrity, in 10%Suc extender, 4%Fructose extender were higher extreme significantly than that in 11% Lac extender（P〈0.01）, and higher significantly than that in 11% Lac in abnormality（P〈0.05）. There was no difference between frozen pellet semen and straw semen in frozen-thawing motility（P〉0.05）.

关 键 词：马 精液保存 稀释液 活率 存活时间 

分 类 号：S821.34[农业科学—畜牧学]