安神补脑液对睡眠剥夺大鼠脑内诱导型一氧化氮合酶及褪黑素的影响  

Effects of Anshen Bunao Syrup on the Inducible Nitric-oxide Synthase Activity and Melatonin Content in Brain of Sleep Deprivation Rats

作　　者：魏海峰[1] 叶翠飞[1] 吴燕川[1] 王玉兰[1] 丁月霞[1] 赵宁[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院,教育部神经变性病重点实验室,北京100053

出　　处：《中药新药与临床药理》2007年第5期369-371,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(90709011;30340090);国家重点基础研究发展规划(973)项目(G2003CB517104);北京市自然科学基金(7032013)

摘　　要：目的研究安神补脑液对睡眠剥夺大鼠脑内诱导型一氧化氮合酶(iNOS)及褪黑素的影响。方法SD大鼠随机分成正常对照组,模型组,安神补脑液高、低剂量组。灌胃给予安神补脑液2周后,用多站台水环境法复制睡眠剥夺模型,取前脑组织测定iNOS的活性,并用高效液相色谱法测定脑内褪黑素的含量。结果与正常对照组比较,睡眠剥夺大鼠脑组织iNOS活力显著增高,褪黑素有减少的趋势。大剂量安神补脑液可使模型大鼠iNOS活力明显降低(P<0.05),褪黑素含量显著提高(P<0.05)。结论安神补脑液可拮抗睡眠剥夺大鼠脑内iNOS活力提高,并能增高松果体褪黑素的含量,提示安神补脑液可以改善睡眠障碍导致的脑功能改变。Objective To study the effects of Anshen Bunao Syrup（ABS） on the inducible nitric - oxide synthase （iNOS） activity and melatonin content in brain of sleep deprivation（SD）rats. Methods Rats were divided into groups of normal control, SD model, SD ＋ low - dose ABS, and SD ＋ high - dose ABS. The rats were fed with ABS for 2 weeks and were subjected to sleep deprivation for 4 days, and then the iNOS activity in the front cortex was analyzed, and melatonin content in the pineal gland was analyzed with HPLC. Results Compared with the normal control rats, the iNOS activity was significantly increased in the front cortex of SD rats and the melatonin content decreased in. Large - dose ABS significantly decreased iNOS activity and increased melatonin content in SD model rats. Conclusion Anshen Bunao Syrup can effectively counteract iNOS activity increase and melatonin secretion disturbance in the brain of SD rats, and can improve the cerebral function of rats with sleep disorder.

关 键 词：安神补脑液 睡眠剥夺 诱导型一氧化氮合酶 褪黑素 大鼠 

分 类 号：R285.5[医药卫生—中药学]