BMP2/7异源二聚体调控CIZ的表达与自身活性的关系  

Relationship Between the Bioactivity of BMP2/7 Heterodimers and Its Regulation of CIZ Expression

作　　者：潘秋辉[1] 杨松海[2] 董群伟[3] 孙奋勇[2] 

机构地区：[1]中山大学附属第二医院医学研究中心,广州510120 [2]暨南大学生命科学技术学院生物工程研究所,广州510632 [3]广东药学院附属第一医院,广州510080

出　　处：《中国生物工程杂志》2007年第9期14-18,共5页China Biotechnology

基　　金：广州市科技计划资助项目(2006Z1-E0031;2006Z3-E0791)

摘　　要：BMP2/7异源二聚体的活性显著高于BMP2同源二聚体,但其机制并不清楚。采用哺乳动物细胞表达的BMP2/7异源二聚体处理成骨细胞MC3T3-E1,细胞化学染色发现BMP2/7的活性显著高于BMP2,报告载体p3GC2-LUX检测发现BMP2/7能够明显上调BMP/Smad通路的活性(P<0.05)。但在成骨细胞中过表达CIZ(casinteracting zinc finger protein),能够显著抑制BMP2/7上调ALP与Osteocalcin的作用,并阻断BMP2/7对BMP/Smad通路的激活。同时发现BMP蛋白能够上调CIZ的表达,但BMP2/7的作用明显低于BMP2同源二聚体。可以认为BMP2/7能够诱导CIZ的表达,但由于作用较弱,所以对自身活性的反馈抑制作用也较弱,这可能是BMP2/7有着较强生物活性的关键所在。BMP2/7 heterodimer has stronger bioactivity than BMP2 homodimer, but the mechanism remains to be unclear. Treating the osteoblast MC3T3-E1 cells with BMP proteins, results of Alkalin phosphatase staining, Alizarin red staining, as well as p3GC2-LUX luciferase assay demonstrated that BMP2/7 heterodimers significantly promoted osteogenic differentiation to a higher extent than BMP2 homodimers. Overexpression of CIZ in MC3T3-E1 significantly inhibited the promotion of ALP and Osteocalcin expression by BMP2/7, and blocked activation of BMP/Smad. BMP protein may induce the expression of CIZ, but effects of BMP2/7 heterodimer is weaker than BMP2 homodimer. It suggests that the effects of BMP2/7 on CIZ expression is related to its bioactivity.

关 键 词：骨形态发生蛋白 异源二聚体 成骨细胞 分化 CIZ蛋白 

分 类 号：Q819[生物学—生物工程]