南方红豆杉组织培养研究进展  

Review on Taxus chinensis var.mairei tissue culture research

作　　者：何康[1] 樊正球[1] 李丽[1] 王祥荣[1] 

机构地区：[1]复旦大学环境科学与工程系生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《中国生态农业学报》2007年第5期215-218,共4页Chinese Journal of Eco-Agriculture

基　　金：复旦大学中青年杰出人才基金项目(EXH1829310)资助

摘　　要：阐述了组培微繁诱苗过程中不同外植体诱导愈伤组织的效果差别、培养基选取和优化、植物生长调节剂浓度和配比、生理碳源、光条件和小分子添加剂等因素的作用影响;从细胞次生代谢诱导和生物反应器应用两方面总结了细胞悬浮培养生产紫杉醇研究现状。此外,对南方红豆杉组织培养的主要问题和方向进行了探讨和展望。Progress on Taxus chinensis var. mairei tissue culture is reviewed in this paper. The paper discusses the effects of explants, culture medium, growth regulator, carbon resources, light condition and small molecular additive on the induction and multiplication of Taxus chinensis var. mairei callus in tissue culture and suggests optimal alternatives. Paclitaxe production is discussed from the view of cell secondary metabolism and application of bio-reactor to Taxus chinensis var. mairei cell suspension culture. Finally, the problems and development direction of Taxus chinensis var. mairei tissue cul- ture research are advanced.

关 键 词：南方红豆杉 组织培养 细胞悬浮培养 紫杉醇 生物反应器 

分 类 号：S791.49[农业科学—林木遗传育种]