T3对MG63细胞株TRAIL,OPG,OPGL表达的影响  

Effects of T3 on expression of TRAIL in MG63 cells

作　　者：周玮[1] 薛国良[1] 王光辉[1] 杜云翔[1] 王加林[1] 张勇[1] 惠亮亮[1] 顾媛[1] 

机构地区：[1]解放军第82医院内分泌科,淮安223001

出　　处：《中国骨质疏松杂志》2007年第9期645-647,共3页Chinese Journal of Osteoporosis

摘　　要：目的观察在不同浓度T3环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其护骨素(OPG)、护骨素配体(OPGL)的表达,探讨甲亢性骨质疏松症的发病机制。方法用不同浓度T3(对照组,10-12、10-10、10-8mol/L组)分别刺激培养的MG63细胞24h,RT-PCR法检测TRAIL,OPG,OPGLmRNA的表达。结果T3对MG63细胞中TRAIL,OPGLmRNA的表达,均按照对照组、10-12mol/L组、10-10mol/L组、10-8mol/L组顺序递增(P<0.05),OPGmRNA的表达按照此顺序递减(P<0.05),10-8mol/L组TRAILmRNA的水平明显高于对照组(P<0.05)。同时,OPG/OPGL比率按照对照组、10-12mol/L组、10-10mol/L组、10-8mol/L组顺序递减。结论T3可能导致成骨细胞中TRAIL和OPGL表达增多,OPG的表达减少。这可能是甲亢性骨质疏松症的重要发病机制之一。Objective To observe the regulative effects of different concentrations of T 3 on the expression of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL）, osteoprotegerin （OPG）, and the ligand of osteoprotegerin（OPGL） in osteosarcoma MG 63 cells, and to study the role of non-physiological concentration of T 3 in pathogenesis of hyperthyrodism osteoporosis. Methods MG 63 cells were incubated with T 3 at the concentration of 10^12, 10^- 10,10^- 8 mol/L for 24 h respectively. The expressions of TRAIL, OPG and OPGL mRNA were examined by reverse transcriptase（ RT）-PCR. Results The mRNA expressions of TRAIL and OPGL in MG 63 cells increased in the order of control group, 10^-12, 10^-10, 10^-8 mol/L T 3 groups（ P 〈 0.05） and the expression of OPG mRNA decreased in the same order（ P 〈 0.05） .TRAIL mRNA level in 10^-8 mol/L group was significantly higher than that in control group[ （ 1.011 ± 0.064） vs （0.736 ± 0.016）, P 〈 0,05）. Meanwhile, the rate of OPG/OPGL decreased in the order of control group, 10^-12 , 10^-10 , 10^-8 mol/L T 3 groups（ P 〈 0.05）. Conclusions High concentration of T 3 could lead to the increasing expression of some bone-resorbing cytokines such as TRAIL and OPGL in osteoblasts but the decreasing expression of OPG, which may be one of the key pathogenetic factors of hyperthymdism osteoporosis.

关 键 词：三碘甲状腺原氨酸 MG63细胞 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R681[医药卫生—骨科学]