骨量、微结构及受力方向对去卵巢大鼠骨生物力学的影响  

Effect of bone mass, microarchitecture and impact direction on biomechanical properties of bone in ovariectomy-induced osterporotic rats

作　　者：褚亚明[1] 姜保国[1] 张殿英[1] 傅中国[1] 徐峰[1] 

机构地区：[1]北京大学人民医院创伤骨科,北京100044

出　　处：《中国骨质疏松杂志》2007年第9期651-654,共4页Chinese Journal of Osteoporosis

基　　金：973基金项目(2005CB522604);北京市科技重大项目培育专项(Z0005190041391)

摘　　要：目的观察骨质疏松模型大鼠骨量、骨微结构和骨的受力方向与生物力学变化的关系。方法6～7月龄Wistar大鼠50只,随机分为正常对照组25只,去势模型组25只,12周后处死全部大鼠,测定大鼠全身、股骨和腰椎(L4～L6)骨密度,对左侧股骨进行三点弯曲试验,右侧股骨沿载荷倾斜20°角进行压力试验,对椎体(L4)进行压缩试验,把部分股骨和腰椎(L5)制成切片进行免疫组化染色,观察它们的形态计量学变化。结果去势组大鼠的全身、股骨和腰椎(L4～L6)骨密度比对照组明显减少(P<0.05),去势组大鼠右侧股骨沿载荷倾斜20°角后的结构力学性能和椎体的最大压缩力较正常对照组明显降低(P<0.05),但两者左侧股骨的结构力学性能差异无显著性(P>0.05),去势后大鼠股骨的材料力学性能较对照组明显下降(P<0.05),组化染色显示去势组大鼠的骨小梁较正常组稀疏减少,尤以松质骨明显。结论大鼠去势后松质骨的骨量、骨微结构较皮质骨变化明显,且骨量、骨微结构和骨的受力方向对骨的生物力学都有着重要影响。Objective To observe relationship between biomechanical change and bone mass, microarchitecture, impact direction in osterporotic rats. Methods Fifty 6-7 month old Wister rats were subjected to 25 for ovariectomy （OVX） and 25 as control. All rats were sacrificed after 12 weeks. Whole body bone mineral density（BMD） and BMD at femur and lumber spine was measured. Biomechanical test was conducted for femur and lumber spine, Partial femur and lumber spines were assayed by immunohistochemical staining. Result BMD in whole body, femur and lumber spines significantly decreased in OVX rats. Compressive load of lumber spines and failure load tested at 20° angle of right femur was significantly lower in OVX rats. But structural mechanics of 0° angle of left femur was not significantly different between two groups. Mechanics of materials significantly decreased in OVX rats. Immunostaining shows that trabecular bone was lost obviously in OVX rats. Conclusion Change of bone mass and microarchitecture was more obvious in OVX rats. Bone mass, microarehitecture, impact direction significantly affect bone biomechanical property.

关 键 词：骨质疏松 生物力学 大鼠 骨微结构 受力方向 

分 类 号：R681[医药卫生—骨科学]