脑铁调素增高诱导膜铁转运蛋白1及其mRNA的表达下调  

Downregulation of ferroportin 1 and its mRNA expression induced by hepcidin

作　　者：陈兵兵[1] 李琳[1] 

机构地区：[1]山西医科大学老年医学研究所,太原030001

出　　处：《山西医科大学学报》2007年第9期793-796,共4页Journal of Shanxi Medical University

基　　金：山西省国际科技合作项目(051015)

摘　　要：目的研究脑室注射铁调素(hepcidin,Hep)对膜铁转运蛋白1(ferroportin1,FP1)及其mRNA表达的调节。方法雄性Wistar大鼠16只,随机分为FP1组及对照组,FP1组脑室注射Hep,对照组脑室注射无菌生理盐水,12h后,分别用免疫组化和RT-PCR法测皮质、海马的FP1及其mRNA的表达。结果脑皮质和海马FP1及其mRNA表达较对照组均明显减少(P<0.01)。结论Hep可直接抑制FP1及其mRNA的表达。Objective To study the expression of FP1 and its mRNA induced by injecting hepcidin（Hep） into lateral cerebral ventricle. Methods Sixteen male Wistar rats were randomly divided into 2 groups： FP1 group（injected with Hep） and control group （injected with sodium chloride injection）. The expression of FP1 and FP1 mRNA in the cortex and hippocampus of rats after injecting into lateral cerebral ventricle for 12 h were measured by immunohistochemistry and RT-PCR. Results The expression of FP1 and its mRNA all decreased significantly in cerebral cortex and hippocampus after injecting Hep（ P 〈 0.01 ） . Conclusion Hep could directly inhibit the expression of FP1 and FP1 mRNA.

关 键 词：铁调素 膜铁转运蛋白1 免疫组化 逆转录聚合酶链反应 

分 类 号：R338[医药卫生—人体生理学]