汉坦病毒体外感染Vero-E6细胞诱导多种热休克蛋白的表达  

The Expression of HSP induced by Hantavirus in Vero-E6 cells in vitro

作　　者：余璐[1] 马恒[2] 段春光[1] 杨守京[1] 

机构地区：[1]第四军医大学病理学教研室,陕西西安710032 [2]第四军医大学生理学教研室,陕西西安710032

出　　处：《医学研究生学报》2007年第9期908-911,I0001,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:39770664)

摘　　要：目的:观察汉坦病毒(HV)体外感染非洲绿猴肾上皮细胞(Vero-E6细胞)诱导热休克蛋白(HSP)的表达并探讨其与病毒结构蛋白的相互关系。方法:采用HV76-118株体外实验性感染Vero-E6细胞,随机分为对照组和病毒感染组,运用免疫细胞化学染色、Western blot及双特异性抗体夹心酶联免疫吸附法(ELISA),观察HV感染后HSP27、HSP70、葡萄糖调节蛋白(Grp94)3种HSP的表达情况,并分析上述HSP与病毒核衣壳蛋白(NP)之间的相互关系。结果:HV76-118株感染Vero-E6细胞24 h后,免疫细胞化学染色法可检出HV-NP阳性信号。Westernblot结果表明,与对照组相比较,HSP27、HSP70、Grp94的表达水平在感染后24 h显著升高(P<0.05,n=5)。更为重要的是,ELISA检测发现HV-NP同时与HSP27、HSP70、Grp94呈复合物形式存在。结论:HV体外感染可诱导Vero-E6细胞高表达多种HSP,且能与病毒结构蛋白形成复合物,上述过程可能参与了HV-宿主细胞的相互作用。Objective：To study heat shock proteins （HSP） and their association with Hantavirus nucleocapsid protein （HV-NP） in Vero-E6 cells infected with Hantaan76-118. Methods： The HV-NP was identified by immunocytochemical staining after infected with Hantaan76-118. The expression of HSP was detected by Western-blot and analyzed by double specific antibody sandwich ELISA. Results： Western- blot exhibited that the expressions of HSP27, HSP70 and Grp94 in the Vero-E6 cells infected with Hantaan 76-118 were significantly higher than those in the control cells （P 〈 0.05, n = 5 ）. More importantly, HSP27, HSP70 and Grp94 were associated with HV-NP （n = 5 ）, which led to the formation of protein complexes. Conclusion ： HV infection induces the expression of a set of HSP including HSP27, HSP70 and Grp94, which are associated with HV-NP. This association may demonstrate the chaperone role of HSP in the hantavirus-host interaction.

关 键 词：汉坦病毒 热休克蛋白 汉坦病毒核衣壳蛋白 

分 类 号：R373.32[医药卫生—病原生物学]