6-BA、NAA对苜蓿不同外植体愈伤组织培养的影响  

Effects of 6-BA and NAA on Different Explants of Cultured Tissues of Alfalfa

作　　者：肖燕[1] 张博[1] 范永刚[2] 王彩虹[1] 赵红娟[1] 王玉祥 

机构地区：[1]新疆草地资源与生态重点实验室/新疆农业大学草业工程学院,乌鲁木齐830052 [2]中国科学院新疆生态与地理研究所

出　　处：《新疆农业科学》2007年第5期658-662,共5页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技厅高新技术项目"利用细胞工程技术培育强优势苜蓿单交种的研究"(200511102)

摘　　要：以新牧1号和新疆大叶两个苜蓿品种为试材,研究了7个培养基对不同无菌苗外植体愈伤组织诱导和生长影响,以及不同浓度的植物生长调节物质6-苄氨基腺嘌呤（6-BA）和萘乙酸（NAA）对愈伤组织生长及分化的作用。结果表明：（1）浓度在2-8 mg/L,NAA有利于苜蓿愈伤组织的诱导,随浓度的增大呈促进作用,而在1-3 mg/L,1mg/L 6-BA有利于苜蓿愈伤组织的诱导,随浓度的增大呈抑制作用;（2）MS＋2,4-D 2mg/L＋NAA 4 mg/L＋6-BA 1 mg/L即MS4为新牧1号的较佳诱导培养基,MS＋2,4-D 2 mg/L＋NAA 8 mg/L＋6-BA 1 mg/L即MS5为新疆大叶苜蓿的较佳诱导培养基;（3）以无菌苗下胚轴、子叶及叶片为外植体,确定下胚轴为诱导愈伤组织的最佳外植体。The paper studied effects of seven kinds of medium on induction and growth of callus, using different of explant of Medicago varia, cv. Xinmu No. 1 and Medicago sativa L. cv. Xinjiang Daye, and effects of different concentration of 6- BA（6- Benzylaminopurine） and NAA（ 1 - Naphthyacetic Acid） on induction, dif- ferentiation callus. The results are as follows： （ 1 ） The concentration of 2 - 8 mg/L, NAA is benefit to induce callus, if it＇s concentration was higher,the callus of alfalfa were better.The concentration of 1 - 3 mg/L, lmg/L 6 -BA is benefit toinduce callus, if it＇s concentration was higher, the callus of alfalfa were worse. （2）MS ＋ 2,4- D 2 mg/L ＋ NAA 4 mg/L ＋ 6 - BA 1 mg/L was the better induction medium of Xinmu No. 1. MS ＋ 2,4 - D 2 mg/L ＋ NAA 8 mg/L ＋ 6 - BA 1 rng/L was the better induction medium of Xinjiang Daye. （3）Using the explant of hypocotyls, cotyledon and leaves as explants, hypocotyls was the best explant of induction callus medium.

关 键 词：苜蓿 愈伤组织 外植体 6-BA NAA 

分 类 号：S551.704.8[农业科学—作物学]