三步层析法分离毕赤酵母工程菌中表达的新疆家蚕抗菌肽  

Isolation and Purification of Cecropin-XJ Expressed in Pichia Pastoris by Three Chromatographic Methods

作　　者：窦君[1] 郑树涛[1] 王娉[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2007年第5期710-714,共5页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高技术研究发展计划项目(200311118);新疆高校创新研究群体基金项目(XJEDU2004G02)

摘　　要：通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽。结果表明,三步层析后,SDS电泳显示单一条带,分子量约为7.0 kDa;所纯化的新疆家蚕抗菌肽具有较强的抗菌活性。该纯化工艺的建立,为新疆家蚕抗菌肽在饲料添加剂、农用抗生素等方面的广泛应用奠定基础。Cecropin- XJ expressed in Pichia pastoris was purified through ion exchange chromatobra. phy （ IEC ）, hydrophobic interaction chromatography （ HIC ） and size exclusion chromatography （ SEC ）. The results showed that there was a single band and its molecular weight was 7.0 kDa. The purified Cecropin- XJ was more active for the antibacterial effects. This purification strategy was established, which will provide a basis for potential application of antibacterial peptide in feed additive and antibiotics used for agriculture, and so on.

关 键 词：新疆家蚕抗菌肽 离子交换层析 疏水作用层析 分子排阻层析 纯化 

分 类 号：S188[农业科学—农业基础科学]