DHA-1型质粒AmpC酶调控因子AmpR基因检测  

Detection of regulator AmpR genes of DHA-1 type plasmid-encoded AmpC β-lactamase in escherichia coli and klebsiella pnuemoniae isolates

作　　者：多丽波[1] 栾英[2] 张联博[2] 李垚[1] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]哈尔滨医科大学附属二院,黑龙江哈尔滨150086

出　　处：《中国实验诊断学》2007年第9期1146-1148,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30672005);黑龙江省博士后基金资助(LBH-Z05030)

摘　　要：目的检测DHA-1型质粒AmpC酶诱导性耐药调控因子AmpR基因。方法以我院临床分离产DHA-1型质粒AmpC酶的4株大肠埃希菌和10株肺炎克雷伯菌为研究对象,采用聚合酶链反应(PCR),用特异性引物扩增Am-pR调节因子全编码基因,并将一株肺炎克雷伯菌AmpR基因克隆到pET-22b(+)载体质粒中测序。结果所有实验菌株AmpR基因检测阳性,重组子AmpR基因测序表明与摩根摩根菌染色体上AmpR基因同源性达99.8%。结论产DHA-1型质粒AmpC酶肺炎克雷伯菌、大肠埃希菌的质粒上存在调控因子AmpR基因。Objective To detect regulator AmpR genes of DHA-1 type plasmid-encoded AmpC β-Lactamnse isolates. Methods AmpR genes of 4 Eseherietfia eoli and 10 Klebsiella pnuemoniae isolates producing DHA-1 AmpC β-Lactamase were analyzed by PCR, AmpR gene of a KlebsielLa pnuemoniae isolate were cloned to pEI22b（ ＋ ） vector and sequenced. Results All of tested strains were AmpR genes positives, the sequence showed that recombinant AmpR gene were highly identical to that of MorganeUa morganii. Conclusion There were reguldator AmpR genes in plnsmid of DHA-1 type AmpC β-Laetamase isolates.

关 键 词：质粒 AMPC酶 调节基因AmpR 序列分析 

分 类 号：R450[医药卫生—治疗学]