乙型肝炎病毒基因型与临床流行病学相关性研究  

Study of correlation between genotype of hepatitis B virus and its clinical epidemiology

作　　者：刘桂艳[1] 周剑惠[1] 许强[2] 王兆南[1] 刘双燕[3] 常新[1] 王爽[1] 陈超[1] 曹凤瑞[1] 张军 田丽敏[5] 

机构地区：[1]吉林省疾病预防控制中心,吉林长春130062 [2]北京大学医学部病原生物学系,北京100083 [3]吉林北方肝胆医院长春,130061 [4]通榆县第二医院,通榆137200 [5]敦化市疾病预防控制中心,敦化133700

出　　处：《中国实验诊断学》2007年第9期1201-1203,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的本研究旨在为乙型肝炎病毒(HBV)的基因型分布与临床流行病学的关系提供证据。方法采用多对型特异性引物套式聚合酶链反应对吉林省194份来自慢性肝炎、肝硬化及肝癌患者中的HBV阳性血清进行基因分型,并采用卡方检验分析基因构成比的差异。结果在194例HBV阳性血清样品中B型16例、C型172例、BC混合型6例,未发现其它型别的病毒。肝硬化与肝癌患者中乙肝病毒的基因型构成比的差异无统计学意义,而慢性肝炎与肝硬化患者中乙肝病毒的基因型构成比的差异有统计学意义,男女患者所携带的病毒基因型差异无统计学意义。结论肝硬化和肝癌患者中C基因型构成比无差异,但二者均高于慢性肝炎患者。男女患者中C基因型构成比无差异。Objective This study is designed to provide evidence for correlation between genotypes of hepatitis B virus and its clinical epidemlology.Methods Genotypes of hepatitis B virus for 194 serum from chronic hepatitis, cirrhosis and hepatocdlular carcinoma cases were classified with multiplex PC.R, and X^2 test was used to analyze the difference of the genotype constitution ratio bettwecn different types of eases.Results 16 genotype B, 172 genotype C and 6 mixture of genotype B and C viruses were detected in the 194 specimens, other genotype were not found. There was no statistical difference of genotype constitution ratio between cirrhosis and hepateeellular carcinoma,and between male and female; but the statistical difference exists between chronic hepatitis and cirrhosis. Conclusion Genotype C constition ratio in cirrhosis and hepaeellular carcinoma are similar, and both of them are higher than which in chronic hepatitis; which is also similar in male and female cases.

关 键 词：乙型肝炎病毒(HBV) 基因型 聚合酶链反应(PCR) 临床流行病学 

分 类 号：R512.6[医药卫生—内科学]