我国犬钩虫ITS及5.8S rDNA的克隆与序列分析  

Cloning and sequence analysis of the ITS and 5.8S rDNA of Ancylostoma caninum in China

作　　者：宋慧群[1] 李宾[1] 林瑞庆[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医科学》2007年第9期756-759,共4页Chinese Veterinary Science

基　　金：国家杰出青年科学基金项目(30225033)

摘　　要：以从我国广东湛江犬小肠中采集的2条犬钩虫作为研究对象,用保守引物NC5及NC2扩增犬钩虫rDNA的ITS-1、5.8S及ITS-2片段,将PCR扩增出的片段纯化后克隆至pGEM-T Easy载体,重组质粒通过菌落PCR和酶切鉴定后,对阳性菌落进行序列测定并进行序列分析。结果显示,来自湛江的2条犬钩虫ITS及5.8S rDNA序列总长均为738 bp,其中ITS-1序列长为364 bp,5.8S序列长为153 bp,ITS-2序列长为221 bp;2个不同样品之间ITS序列存在多态性,ITS-1序列存在5个碱基的差异,ITS-2序列存在1个碱基的差异,5.8S rDNA序列无差异。研究结果为犬钩虫进一步的分类、鉴定和遗传变异研究奠定了基础。The internal transcribed spacer（ITS） and 5.8S rDNA of Ancylostoma caninum isolated in Guangdong Province of China were amplified by PCR using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector, respectively. The inserts were successfully sequenced,and the ITS of the two A. caninum samples was 738 bp in length. Sequence analysis revealed that the ITS-1,5.8S and ITS-2 rDNA of both A. caninum samples were 364 bp,153 bp and 221 bp in length,respectively,and poly morphism was found in the ITS-1 and ITS-2 rDNA sequences between the two A. caninum samples. The results provided a foundation for further studies of molecular identification and molecular genetics of A. caninum.

关 键 词：犬钩虫 内转录间隔区(ITS) 5.8S RDNA PCR 序列分析 

分 类 号：S852.731[农业科学—基础兽医学] Q78[农业科学—兽医学]