O型口蹄疫疫苗免疫牛抗体消长动态的LPB-ELISA检测  

Dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine by the liquid-phase blocking ELISA

作　　者：魏孔福[1] 祁淑芸[2] 林密[2] 冯霞[2] 张峰[1] 马军武[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2007年第9期787-790,共4页Chinese Veterinary Science

基　　金：国家"十五"科技攻关项目(2002BA5114A-18-1)

摘　　要：分别按一次免疫、首免后第15 d加强免疫和首免后第60 d加强免疫程序,对3组试验牛(每组牛20头)用O型口蹄疫疫苗进行了免疫。免疫后不同时间点用液相阻断ELISA(LPB-ELISA)测定了免疫牛O型口蹄疫抗体效价。结果,首免后第15 d加强免疫牛和第60 d加强免疫牛抗体水平较高,50%保护牛所占比例分别为56.3%和63.1%,完全受保护的牛所占比例分别为34.3%和29.5%。第60 d加强免疫牛的保护率要高于第15 d加强免疫牛和一次免疫牛的保护率。结果表明,首免后第60 d加强免疫是最理想的免疫程序。Three groups（n=20） of cattle were vaccinated with the foot-and-mouth disease（FMD） type O vaccine according to the protocol of primary immunization（PI）and the booster-immunization on day 15 and 60 post-PI,respectively. Titers of the antibody against FMDV type O were detected with the liquidphase blocking ELISA（LPB-ELISA）on different days post-PI or post-booster-immunization. Levels of the antibody in the cattle re-vaccinated on days 15 and 60 post-PI were higher than that in the primarily-immu- nized cattle,the cattle belonging to protection range of 50% made up 56.3% and 63.1%, and the cattle belonging to protection range of 100% made up 34.3% and 29.5% ,respectively. The results showed that the booster-immunization on day 60 post-PI was the most favorable procedure.

关 键 词：O型口蹄疫 液相阻断ELISA 抗体检测 抗体消长 

分 类 号：S852.659.6[农业科学—基础兽医学]