家蚕脂肪体细胞原代培养及其细胞分化的研究  

Study on Primary Culture and Differentiations of Fat Body Cells From Bombyx mori

作　　者：潘敏慧[1] 刘敏[1] 蔡秀娟[1] 洪开丽[1] 许湲[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与系统生物学研究所农业部蚕桑学重点开放实验室,重庆400716

出　　处：《蚕业科学》2007年第3期403-408,共6页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB121-000);国家高技术研究计划"863"项目(编号2006AA10A-117);国家自然科学基金项目(编号30471312)

摘　　要：取家蚕4~5龄幼虫背部脂肪体,采用胰蛋白酶消化和组织块贴壁法进行离体培养,观察脂肪体细胞在体外培养过程中的变化。家蚕脂肪体细胞在原代培养过程中出现了一批具有繁殖和再分化能力的脂肪前体细胞,细胞间呈管道状或网状排列,可在体外培养条件下繁殖,分化成新的脂肪组织,形成的脂肪细胞分泌大量脂肪油滴后破裂死亡。研究结果可为进一步探讨家蚕脂肪体细胞的分化及脂类代谢调节机制提供实验条件。The dorsal fat bodies of silkworm larva in 4^th trypsin-digest method and tissue pieces-stick method. and 5^th instars were obtained and cultured in vitro with During the course of differentiation of precursor lipocytes from silkworm, their morphological changes were observed and a group of precursor lipocytes which had capability of propagation and cell differentiation appeared. They were pipeline-like or reticulately arranged and could grow, differentiate and regenerate into new fat bodies in vitro culture, finally the lipocytes ruptured to death after secreting a great deal of lipid droplets. The results will provide the experimental condition for study of differentiation of fat body cells and mechanism of lipid metabolism of Bombyx mori.

关 键 词：家蚕 脂肪体细胞 原代培养 分化 

分 类 号：Q813.11[生物学—生物工程]