西瓜种质资源的遗传多样性及亲缘关系的AFLP分析  

Study on Genetic Diversity and Relationship in Watermelon Germplasms by AFLP Analysis

作　　者：李艳梅[1] 段会军[1] 马峙英[1] 

机构地区：[1]河北农业大学农学院,河北保定071001

出　　处：《华北农学报》2007年第B08期177-180,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(303188);河北省教育厅科研基金资助项目(2002141)

摘　　要：利用AFLP指纹图谱分析技术对57个西瓜材料进行了基因组DNA多态性分析。结果表明,18对AFLP引物共产生709条DNA带,平均每个引物组合扩增出39.4条带,其中440条为多态性带,占总带数的61.4%;根据DNA谱带计算品种间遗传相似系数,其变化范围在0.24-0.99之间,平均相似系数为0.70,说明西瓜品种间遗传基础狭窄。用NTSYS软件进行聚类分析,57个西瓜品种被划分为2个类群。其中类群1包括1个野生西瓜,类群2均为栽培种。在类群2中引进的资源聚在一起,杂交种和双亲或其一聚在一起。此外,本研究表明,AFLP指纹分析技术具有很高的分辨率,能够揭示西瓜品种间的差异,是鉴定西瓜品种的有力工具。57 watermelon materials were analyzed using AFLP. The results showed that 709 AFLP bands were amplifled using 18 primer pairs, among them, 440 （61.4 % ） bands were polymorphic. Based on DNA bands, the similarity coefficient were calculated and their average value was 0.70, ranging from 0.24 to 0.99, which indicated that relationship was close and their genetic base was narrow among materials of watermelons. When all of the polymorphic bands were analyzed by cluster analysis, 57 watermelon materials were classified into 2 groups. Group included 1 wild watermelon material, and group,56 watermelon materials fell into it. Among the group introduced watermelon materials were clustered together as well as the F1 and parent materials. In addition, this study demonstrates that AFLP fingerprinting has higher resolution and can reveal the difference of cultivars, so it is an efficient tool.

关 键 词：西瓜 品种 遗传多样性 亲缘关系 AFLP分析 

分 类 号：S651.01[农业科学—果树学]