盾壳霉产葡聚糖酶培养条件的研究  

Study on Culturing Condition of Glucanases Produced by Coniothyrium minitans

作　　者：李欣[1] 高俊明[1] 马丽娜[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《华北农学报》2007年第B08期185-188,共4页Acta Agriculturae Boreali-Sinica

基　　金：山西省自然科学基金资助项目(20041096)

摘　　要：通过在SMCS培养液中加入不同的碳源、氮源,改变培养时间、温度及培养液的pH值,研究了盾壳霉产葡聚糖酶的培养条件。结果表明:盾壳霉接种在改良的SMCS液体培养基中置于恒温摇床(200 r/min,20℃)上培养15 d,可以诱导产生大量的葡聚糖酶。改良的SMCS培养液配方为:KH2PO4680 mg,K2HPO4870 mg,KCl 200 mg,NH4NO31 g,Mg-SO4.7H2O 200 mg,CaCl2200 mg,FeSO42 mg,ZnSO42 mg,MnSO42 mg,蔗糖10 g,加水1 000 mL,调pH值到6.5。In this study through the different carbon source, the nitrogen source and the double carbon source are mixed in the SMCS nutrient fluid. The culturing time, the temperature and the pH are changed in order to study culturing condition of glucanases produced by Coniothyrium minitans. The result indicated that, Coniothyrium minitans on the improved SMCS nutrient fluid at the constant temperature on the skakier（200 r/min,20℃） about 15 d,may induce produces massive glucanases. The improved SMCS nutrient fluid formula is：KH2PO4 680 mg, K2HPO4 870 mg, KCl 200 mg, NH4NO3 1 g, MgSO4·7H2O 200 mg,CaCl2 200 mg,FeSO4 2 mg,ZnSO4 2 mg,MnSO4 2 mg, saccharose 10 g,water 1 000 mL,adjusting the pH to 6.5.

关 键 词：盾壳霉 葡聚糖酶 培养条件 

分 类 号：Q814[生物学—生物工程]