小白菜小孢子胚状体诱导影响因素研究  

Embryogenesis from Isolated Microspore Culture of Chinese Cabbage(Brassica campestris L.ssp.chinensis L.)

作　　者：王超楠[1] 冯辉[1] 姜凤英[2] 刘如娥[1] 周英[1] 

机构地区：[1]沈阳农业大学国艺学院,辽宁沈阳110161 [2]辽宁省农业科学院花卉研究所,辽宁沈阳110161

出　　处：《华北农学报》2007年第B08期204-206,共3页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30671414);国家863项目(20060110Z1072)

摘　　要：以10个小白菜杂交种为试材,采用游离小孢子培养方法,研究影响小孢子胚状体形成因素的结果表明,不同品种胚状体的诱导率不同;NLN培养基中大量元素减半有利于小孢子胚诱导,其诱导率达到100%;培养基中添加0.05 mg/L的6-BA和0.2 mg/L的NAA可以提高胚状体诱导率;活性炭和琼脂糖对诱导胚状体发生和发育有促进作用,可使子叶胚的比例达90%以上;花蕾经4℃低温预处理24 h后的成胚率显著提高。Isolated microspore culture was carried out by ten F1 hybrids of Chinese cabbage （ Brassica campestris L. ssp. chinensis L. ）. It was found that the effect of genotypes on embryoid induction was remarkable. Reducing the content of major salts of NLN medium by half can raise the embryoid induction rate up to 100% ; the suitable concentration of 6- BA and NAA can remarkably enhance microspore embryogenesis. Agarose and active carbon could not only promote embryogenesis, but also increase the proportion of cotyledon-shaped embryoids, the cotyledon-shaped embryoid rate was over 90 %. 4 ℃ pretreatment of bud for 24 h significantly enhanced frequency of embryoids.

关 键 词：小白菜 游离小孢子培养 胚状体 

分 类 号：S634.3[农业科学—蔬菜学]