大白鼠海马CA_1区锥体细胞的急性分离及其电生理学特性  

ACUTE ISOLATION AND ELECTROPHYSIOLOGICAL PROPERTIES OF CA_1 PYRAMIDAL NEURONS IN HIPPOCAMPUS OF RATS

作　　者：张宗明[1] 吴才宏[2] 阳冬梅[2] 庄道斌[2] 周培爱[2] 周孝思[1] 

机构地区：[1]北京医科大学第三医院外科,100083 [2]北京大学生物膜与膜生物工程国家重点实验室

出　　处：《北京医科大学学报》1997年第1期5-7,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金!39500142;中国博士后科学基金

摘　　要：目的：建立适用于膜片组技术（Patchclamptechnigue，PCT）的大白鼠海马CA_4区锥体细胞（rathippocampalCApyramidalneuron，RHCPN）的急性分离方法，并测定该细胞的电生理学特性。方法：用7～21d大鼠，以链霉蛋白酶E酶解加吸管吹打法制备RHCPN，用全细胞PCT测定其电生理学特性。结果：分离的RHCPN易用于PCT，且具有正常的电生理学特性，并保存了主要的电压依赖性和受体门控性高于通道特性。结论：本法分离的RHCPN适用于PCT电生理学研究。To acutely isolate rat hippocampal CA1 pyramidal neurons (RHCPN) suitable forpatch clamp technique (PCT), and to measure their electrophysiological properties. Methods:RHCPNwere obtained by pronase E treatment and then by trituration with pipette from 7 to 21 day-old rats.PCT was used to study their electrophysiological properties. Results:The isolated RHCPN were easy torecord by PCT. Their electrical and chemical membrane preperties concerning some major voltage -de-pendent and receptor-gated ionic channels were well-preserved. ConcIusion: The RHCPN isolated bythis method is suitable for PCT electrophysiological study.

关 键 词：海马 锥体细胞 分离 电生理学 

分 类 号：R338.26[医药卫生—人体生理学]