中西药结合治疗腹腔感染大鼠血浆肿瘤坏死因子-α和白细胞介素-6的变化  

Change in plasma tumor necrosis factor-α and interleukin-6 levels after intraperitoneal infection under treatment of combining antibiotics with traditional Chinese medicine in rat

作　　者：黄法江 陈建青[1] 潘一民[1] 刘彩虹[1] 黄长河[1] 

机构地区：[1]浙江省浦江县人民医院,浦江322200

出　　处：《感染．炎症．修复》2007年第2期95-97,共3页Infection Inflammation Repair

摘　　要：目的:观察抗生素联合中药治疗对腹腔感染时血浆炎症因子水平的影响,为治疗腹腔感染提供新策略。方法:选取雄性SD大鼠100只,随机分为假手术组、模型组、泰能组(泰能20mg/kg)、血必净组(血必净8ml/kg)、联合用药组(泰能20mg/kg+血必净8ml/kg)5个组,每组20只。采用盲肠结扎穿孔诱发腹腔感染模型,从尾静脉注入不同药物,给药后2h、6h从股静脉抽取静脉血2·5ml,离心后测定血清肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)浓度。结果:模型组血浆TNF-α水平2h较6h高;泰能组与模型组比较,2h较低(P<0·01),6h无显著性差异(P>0·05);血必净组和联合用药组明显低于模型组和泰能组(P<0·01)。模型组血浆IL-6水平明显高于假手术组(P<0·01),各治疗组均较模型组低(P<0·01),而联合用药组又较血必净注射液或泰能组更低(P<0·01)。结论:联合应用中药血必净和西药泰能治疗腹腔感染,能更有效地降低血浆TNF-α、IL-6水平,是治疗腹腔感染的新策略。Objective： To investigate the change in plasma levels of pro-inflammatory mediators during the treatment of intra-peritoneal infection with traditional Chinese medicine and antibiotics simultaneously in rat,in order to find a new treatment strategy of intra-peritoneal infection. Method： One hundred male SD rats were divided into five groups randomly： sham group, control group, Imipenem group （ 20 mg/kg）, Xuebijing （ 血必净） group （8 ml/kg） ,combination group（Imipenem 20 mg/kg＋Xuebijing 8 ml/kg）, with 20 rats in each group. Cecum ligation and puncture （CLP） was done to produce intra-peritoneal infection . Each group was further divided into two sub-groups, the treatment was begun either 2 hours or 6 hours after CLP, 2.5 ml of blood were taken from the femoral vein. The plasma levels of tumor necrosis factor-α（TNF-α）and interleukin-6 （IL-6）were measured after centrifugation. Results：Plasma TNF-α level in control group peaked 2 hours after CLP and decreased at 6 hours. It was lower in antibiotics group than in control group （P〈0. 01）, and there was no difference 6 hours after operation （P〈0.05）. In Xuebijing group and combination group TNF-α levels were significantly lower than in control group and antibiotic group （P〈0. 01）. Plasma IL-6 level in control group was significantly higher than in sham group （P〈0.01）. Plasma IL-6 levels were lower in all the other groups compared with control group, and that in combinationgroup was lower than in antibiotics or Xuebijing group （P〈0.01）. Conclusion： The combination of Xuebijing and antibiotics （Imipenem） for the treatment of intra-peritoneal infection could decrease the plasma levels of TNF-α and IL-6.

关 键 词：腹腔感染 全身炎症反应综合征 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R593.22[医药卫生—内科学]