急性放射性皮肤溃疡形成早期表皮生长因子的表达及意义  

The changes and significance in epidermal growth factor expression in acute radiation-induced skin ulcers

作　　者：丁宏举 曹卫红[2] 谷庆阳[3] 杨志祥[3] 王德文[3] 

机构地区：[1]山东省胶州市中医院急诊科,胶州266300 [2]解放军总医院第一附属医院全军烧伤研究所,北京100037 [3]军事医学科学院放射医学研究所,北京100850

出　　处：《感染．炎症．修复》2007年第3期155-157,F0002,共4页Infection Inflammation Repair

摘　　要：目的:探讨急性放射性皮肤溃疡形成早期表皮生长因子(EGF)的表达和意义。方法:以50Gy^(60)Coγ射线单次局部照射建立急性放射性皮肤溃疡动物模型,以手术方法建立单纯皮肤伤口动物模型,采用免疫组化、原位杂交等方法检测伤口及溃疡组织内EGF的表达。结果:创伤组伤后5～10d,肉芽组织内EGF表达呈强阳性;照射后21～28d,放射性溃疡肉芽组织内EGF表达呈弱阳性,较创伤后5～10d明显减弱。结论:辐射诱导的肉芽组织内EGF表达减弱与急性放射性皮肤溃疡愈合延迟有关。Objective： To study the changes and significance of epidermal growth factor （EGF） expression in early stage of acute radiation-induced skin ulcers. Methods： A model of normal skin wounds was reproduced. Another model of skin ulcer was reproduced with 50 Gy^ 60Co V-rays. Immunohistochemical staining, in situ hybridization （ISH）were used to detect the expression of EGF in simple skin wounds and acute radiation-induced skin ulcers. Results：In simple skin wounds group, the expression of EGF was strongly positive in granulation tissues on 5 to 10 days after injury; while in radiation group, the expression of EGF was weaker in granulation tissues on 21 to 28 days after radiation, compared with simple skin wounds group on 5 to 10 days after injury. Conclusion： Radiation-induced reduction of EGF is relevant to the wound healing retardation of acute radiation-induced skin ulcers.

关 键 词：放射性皮肤溃疡 创面愈合 生长因子 

分 类 号：R818.895[医药卫生—放射医学]