FQ—PCR检测HBV携带者精液、前列腺液HBV—DNA结果分析  

Results analysis of HBV-DNA in semen and prostatic fluid in patients with chronic hepatitis B virus by FQ-PCR

作　　者：孙振亚[1] 郑定容[1] 叶健翔[1] 

机构地区：[1]深圳市宝安区西乡人民医院检验科,518102

出　　处：《中国医师进修杂志》2007年第9期1-3,共3页Chinese Journal of Postgraduates of Medicine

摘　　要：目的通过检测HBV携带者的血清、前列腺液和精液中乙型肝炎病毒（HBV）-DNA含量，研究三者的关系及其临床意义。方法选择经血清学检测诊断为男性HBV携带者120例，采用荧光定量聚合酶链反应（FQ—PCR）技术测定其血清、前列腺液和精液中HBV—DNA含量，并对所有检测指标进行相关性分析。结果120例携带HBV男性中，HBsAg、HBeAg、HBcAb三项阳性者的血清、前列腺液和精液中HBV—DNA的阳性检出率均为100．0％；HBsAg、HBeAb、HBcAb三项阳性组中阳性率分别为86．0％、83．7％和83．7％；HBsAg、HBcAb两项阳性组中的阳性率分别为87．2％、82．1％和84．6％。精液HBV—DNA含量与血清HBV—DNA含量呈正相关（r=0．896），且血清HBV—DNA含量高于精液含量10倍左右：前列腺液HBV—DNA含量与血清HBV—DNA含量呈正相关（r=0．893），且血清HBV-DNA含量高于前列腺液含量10倍左右；前列腺液HBV—DNA含量与精液HBV—DNA含量呈正相关（r=0．993），且前列腺液HBV—DNA含量与精液含量比较差异无统计学意义。结论精液、前列腺液与血清三者中HBV—DNA含量呈正相关。血清HBV—DNA阳性的育龄男性应做精液、前列腺液HBV—DNA检测，以对其阳性者采取防治措施，其性伴侣也应加强防护并进行预防接种。Objective To examine the hepatitis B virus （HBV）-DNA content in serum, prostatic fluid and semen in patients with chronic HBV and study the relationships with them and their clinical significance. Methods Serum,prostatic fluid and semen HBV-DNA of 120 cases HBV infection were detected by fluorescent quantitative polymerase chain reaction（FQ-PCR） and analyzed the relationships. Resuits In the 120 cases, the HBV-DNA positive rate of HBsAg, HBeAg, HBcAb positive samples in serum, semen and prostatic fluid were all 100.0%. The HBV-DNA positive rate of HBsAg, HBeAb,HBcAb positive samples in serum, semen and prostatic fluid were 86.0%, 83.7% and 83.7%. The HBV-DNA positive rate of HBsAg, HBcAb positive samples in serum,semen and prostatic fluid were 87.2% ,84.6% and 82.1%. The amount of HBV-DNA in serum and in semem had positive relationship （r = 0.896） and the amount of HBV-DNA in serum was higher than that in semen about 10 times. The amount of HBV-DNA in prostatic fluid and in serum had the positive relationship（r = 0.893 ） and the amount of HBV-DNA in serum was higher than that in prostatic fluid about 10 times .The amount of HBV-DNA in semen and in prostatic fluid had the positive relationship（r = 0.993 ） and the amount of HBV-DNA in prostatic fluid and in semen had no significant difference. Conclusions The amount of HBV-DNA in semen,prostatic fluid and.serum has the positive relationships. The male whose HBV-DNA is positive in serum must be detect the amount of HBV-DNA in semen or prostatic fluid and do something to defend. His sex-companion should be inoculated with a HBV vaccine.

关 键 词：乙型肝炎病毒 精液 前列腺液 荧光定量聚合酶链反应 

分 类 号：R4[医药卫生—临床医学] R51