谷子胚性愈伤组织粘液提取物对原生质体培养的影响  

The Effect of Mucilaginous Substances Extracted from Foxtail Millet Embryogenic Callus Cultures on Its Protoplast Cultures

作　　者：董晋江 夏镇澳[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《植物生理学报（0257-4829)》1997年第1期73-76,共4页Acta Phytophysiologica Sinica

摘　　要：将谷子胚性愈伤组织粘液提取物添加到谷子原生质体培养基中，其对原生质体培养的影响表明该提取物有助于原生质体形成细胞壁；并且该类有粘液分泌的念伤组织的原生质体游离所需的酶液浓度低、处理时间短。由原生质体形成完整细胞的数量在一定范围内与谷子原生质体培养的植板率相对应；通过增加形成完整细胞的数量可较大幅度地提高原生质体培养的植板率。Embryogrnic callus cultures of cereal crops are usually accompanied by the excrehon of mucilaginous substances, but the composihon and function of these substances are yet to be analyzed. In this paper, we reported the isolation of protoplasts from embryogenie calli with mucilage by brief enzyme areatment, and the protoplast isolahon was greatly facilitated by coupling moderate mechanic application (Figs. 1, 2 ). We added the mucilage extract from embryogenic callus of foxtail thellet (Setaria italica L. Deauv. )to the media for culture of its protoplasts,to assess the effect of the extract on protoplast culture. The results showed that the addihon of the extract promoted cell wall formation by protoplasts.Within the range of test,the number of the intact cells formed from protoplasts was correlated with the plating efficiency of protoplast culture, which was greatly enhanced by the addition of the mucilage extract to the protoplast culture media (Table 1 ).

关 键 词：谷子 原生质体培养 粘液提取物 愈伤组织 

分 类 号：S515.01[农业科学—作物学]