交叉培养法研究养分对作物幼苗释放N_2O的影响  

Effect of nutritional condition on N_2O emission by crop seedlings——a sand liguid cross culture study.

作　　者：陈欣[1] 沈善敏[1] 张璐[1] 吴杰[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110015

出　　处：《应用生态学报》1997年第2期177-180,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金! ( 3 9570 1 3 2 );中国科学院陆地生态系统痕量物质生态过程开放实验室资助

摘　　要：为验证土壤中N2 O能否通过植物的输导组织由叶片释放从而干扰植物直接释放N2 O的定量测定 ,实验采用分室隔离法确证了土壤N2 O对测定某些作物 (如玉米、高粱 )直接释放N2 O的干扰 ;采用交叉培养法消除了这种干扰 ,准确地测定了大豆、玉米幼苗直接释放N2 O的强度 ;并研究了N、P营养与植物释放N2 O及体内NO 3 N浓度之间的相关性 .With separated compartment culture technique, the study confirmed that N 2O in soils has a great interference on the measurement of N 2O emitted directly by some plants such as maize and sorghum. The interference can be effectively eliminated by means of sand liquid cross culture technique. With this technique, the intensity of N 2O emitted directly by seedlings of both soybean and maize is accurately determined, and the correlations between N and P nutritions on the one hand and N 2O emission and NO 3 N concentration of crop seedlings on the another hand are obtained.

关 键 词：作物生态学 幼苗 一氧化二氮 释放 交叉培养法 

分 类 号：S314[农业科学—作物栽培与耕作技术]