红藻条斑紫菜R-藻红蛋白晶体的时间分辨偏振荧光研究  

Studies on the Time Resolved Polarized Fluorescence of R Phycoerythrin Crystal from Red Alga Porphyra yezoensis

作　　者：郑锡光[1] 汪河洲[1] 赵福利[1] 余振新[1] 朱晋昌[2] 张季平[3] 

机构地区：[1]中山大学超快速激光光谱学国家重点实验室 [2]中国科学院感光化学研究所 [3]中国科学院生物物理研究所生物大分子国家重点实验室

出　　处：《生物化学与生物物理学报》1997年第2期206-212,共7页

基　　金：国家自然科学基金;广东省自然科学基金;中山大学前沿科学基金

摘　　要：本文在皮秒时域内研究红藻条斑紫菜Ｒ－藻红蛋白单晶在不同晶轴取向下的偏振荧光动力学过程。荧光衰减表现为二指数过程，即与色素之间的能量传递和激发平衡有关的快过程（τ１≤６０ｐｓ）和色素荧光跃迁过程（τ２～３００ｐｓ）；由于色素间的能量传递，荧光明显退偏振；由于晶体中色素的光学跃迁矩取向倾向于沿主晶轴方向分布，在不同的晶轴的取向下各向异性荧光衰减过程明显不同。The time resolved polarized fluorescence of oriented R phycoerythrin single crystal from red alga Porphyra yezoensis was studied in picosecond time scale. The fluorescence decay of the crystal exhibited two exponential processes, one of which was related to the energy transfer and excitation equilibrium between chromophores (τ 1≤60 ps), and the other to the fluorescent transition of chromophores (τ 2≈300 ps).The fluorescence of the crystal was highly depolarized due to excitation transfer between chromophores, and its anisotropic decay was distinctly dependent upon the crystal orientation because of the distribution of chromophore optical transition moments predominantly orienting around the principal axis of the crystal.

关 键 词：红藻 条斑紫菜 R-藻红蛋白 晶体 偏振荧光 

分 类 号：Q949.292.1[生物学—植物学]