兔肺内小动脉平滑肌细胞培养  

CULTIVATION OF INTRA PULMONARY ARTERIOLAR SMOOTH MUSCLE CELL FROM RABBIT

作　　者：胡德辉[1,2] 刘惠君[1,2] 马传桃[1,2] 

机构地区：[1]湖南医科大学心血管生理研究室 [2]第一军医大学生理教研室

出　　处：《中国应用生理学杂志》1997年第1期80-82,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：肺内小动脉用低浓度胰蛋白酶从肺动脉灌洗后，分离并剪碎，反复用培养基清洗。将沉淀的组织块行肺内小动脉平滑肌细胞培养。培养的细胞经光镜、透射电镜及免疫组化验证为典型的平滑肌细胞。此法简单、方便，可推广应用于各种小血管平滑肌细胞培养，为阻力血管、容量血管疾病的研究提供了理想的实验材料。By perfusion with trypsin at low concentration, the intra pulmonary arterioles were separated carefully and cut into fine tissue pieces. These pieces were then washed with culture medium and used for culture of smooth muscle cells by method of adhesion to wall. Cells cultured were typical smooth muscle cells under light microscope, electron microscope and immunohistochemical test. The method is simple and convenient.It is also applicable to culture of vascular smooth muscle cells from other organs and provides ideal materials for vascular physiopathologic studies.

关 键 词：肺动脉高压 肺内小动脉 平滑肌细胞 细胞培养 

分 类 号：R543.202[医药卫生—心血管疾病]