禾谷镰孢原生质体的形成与再生  

FORMATION AND REGENERATION OF PROTOPLASTS FROM FUSARIUM GRAMINEARUM Schw.

作　　者：徐敬友[1] 胡广淦[1] 童蕴慧[1] 李清铣[1] 

机构地区：[1]江苏农学院植保系

出　　处：《江苏农学院学报》1997年第2期71-73,共3页Jiangsu Agricultural Research

基　　金：江苏省教委自然科学基金

摘　　要：研究报道了制备禾谷镰孢（ＦｕｓａｒｉｕｍｇｒａｍｉｎｅａｒｕｍＳｃｈｗ．）原生质体的方法及影响原生质体再生的条件。ＰＤＳ培养液中培养的菌丝和５％绿豆汁中产生的分生孢子，在２８℃下通过蜗牛酶（３％）、蜗牛酶＋纤维素酶＋溶菌酶（１．５％＋１．５％＋１．５％）酶解，２ｈ开始有原生质体释放，５ｈ左右达释放高峰，后趋于稳定。而以纤维素酶（３％）、溶菌酶（３％）、蜗牛酶＋纤维素酶（１．５％＋１．５％）、蜗牛酶＋溶菌酶（１．５％＋１．５％）、纤维素酶＋溶菌酶（１．５％＋１．５％）处理，原生质体形成效果较差。原生质体在ＰＤＡＳ高渗培养基上再生频率较高（２０％～３０％），在ＭＭＳ上再生频率较低，而在ＮＭＳ上原生质体则不能再生。The preparation and regeneration of protoplasts from Fusarium graminearum Schw. were reported in this paper. By using snail digestase or a combined enzyme system containing commercial cellulase, snail digestase and lysozyme at 28℃, protoplast preparation was obtained from mycelia and conidia. High regeneration frequency of protoplasts was observed on PDAS plates while low regeneration frequency of protoplasts on MMS plates. No regeneration of protoplasts was found on NMS plates. The regeneration of protoplasts was markedly affected on the PDAS amended with KClO 3 or MNNG.

关 键 词：禾谷镰孢 原生质体形成 原生质体再生 

分 类 号：S432.44[农业科学—植物病理学]