RAPD在玉米类群划分研究中的应用  

Heterotic Grouping of 15 Maize Inbreds with RAPD Markers

作　　者：刘新芝[1] 彭泽斌[1] 傅骏骅[1] 李连城[1] 黄长玲 

机构地区：[1]中国农业科学院作物育种栽培研究所

出　　处：《中国农业科学》1997年第3期44-51,共8页Scientia Agricultura Sinica

基　　金：国家攀登计划资助

摘　　要：利用ＲＡＰＤ分子标记，对我国目前推广杂交种的１５个主要亲本自交系进行了类群划分。从１０５个引物中筛选出对１５个不同基因型的玉米自交系扩增产物具有多态性的引物６个，它们是Ｅ－１７、Ｇ－１４、Ｆ－１４、Ｎ－１８、Ｎ－０６、Ｇ－０４，依据６个引物扩增谱带建立０、１型数据，计算１５个自交系间遗传距离，并进行聚类分析。结果表明：供试的１５个自交系共划分为６个类群：Ⅰ类为中黄２０４、关１７、Ｍｏ１７，属兰卡斯特血缘；Ⅱ类为Ｄ黄２１２、黄早四、汶黄，属塘四平头血缘；Ⅲ类为Ｂ３７、Ｂ７３、Ｂ８４，属瑞德黄马牙血缘；Ⅳ类为旅９、Ｅ２８、丹３４０，属旅大红骨系统；Ⅴ类为５００３、４７８；Ⅵ类为自３３０。试验结果进一步表明：用ＲＡＰＤ标记进行玉米自交系类群划分与系谱法一致。Heterotic groups were classified by cluster analysis with RAPDs based on 15 maize inbreds utilized widely in hybrid maize production in China. Six primers, E 17, G 14, F 14, N 18, N 06 and G 04, selected from 105 candidate primers act as polymorphic DNA (RAPD) markers for 15 inbred lines. Genetic distance among 15 inbreds was calculated and clustered with RAPD markers. The results showed that 15 inbred lines could be classified into six groups. Group I consisting of Zhonghuang204, Guan 17 and Mo17, is included in the Lancaster heterotic group. GroupⅡ, Consisting of D Huang 212, Huangzaosi and Wenhuang, is included in the Sipingtou heterotic group. GroupⅢ consisting of B37, B73 and B84, is included in the Reidyellowdent heterotic group. GroupⅣ consisting of Lü9, E28 and Dan340, is included in the Lüdahonggu heterotic group. GroupⅤ contains 5003 and 478, and GroupⅥ contains Zi 330. The six groups classified by cluster analysis with RAPD markers is consistent with the expected grouping based on available pedigree. The current study indicated that it is practical to group maize inbred lines with RAPDs.

关 键 词：玉米 自交系 RAPD 聚类分析 类群划分 

分 类 号：S513.032[农业科学—作物学]