IBRV DQ分离株gI基因的克隆与序列分析  

Cloning and Sequence Analysis of gI Gene of Infectious bovine rhinotracheitis virus DQ Strain

作　　者：王延涛[1] 周玉龙[1] 李国军[1] 侯喜林[1] 朴范泽[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《动物医学进展》2007年第9期23-25,共3页Progress In Veterinary Medicine

基　　金：黑龙江省研究生科研创新资金项目(YJSCX2007-0254HLJ)

摘　　要：通过PCR扩增得到牛传染性鼻气管炎病毒大庆分离株（IBRV-DQ）gI基因上一个398 bp的片段.扩增产物经克隆、测序,所得到的核苷酸序列与GenBank上已发表的此病毒其他分离株的核甘酸序列相比,其同源性在95.5%～99.3%,表明IBRV gI基因非常保守.在系统进化树中IBRV-DQ株与U14106.1、U14107.1株亲缘关系较近,属于同一个分支.The gI gene of infectious bovine rhinotracheitis virus（IBRV） Daqing strain （IBRV-DQ） was amplified through PCR . The amplification product consisted of 398 bp. After being cloned and sequenced , the homology of the nueleotide sequence was 95.5%-99.3% comparison with the published IBRV-gI gene sequence of GenBank. The results indicated that gI gene of the IBRV-DQ strain was relative conservative. Phylogenetie tree analysis showed that the IBRV-DQ strain had high homology to the U14106. land U14107.1, and they belonged to the same branch.

关 键 词：牛传染性鼻气管炎病毒 IBRV-DQ株 GI基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]