微生物基因芯片鉴定及再生利用  

Microbe identification using gene chip and reapplication

作　　者：黄国亮[1] 徐书宽[1] 邓橙[1] 朱疆[1] 杨阳[1] 董中华[1] 李英姿[2] 阳小勇[3] 张亮[3] 

机构地区：[1]清华大学医学院,北京100084 [2]清华大学生物科学与技术系,北京100084 [3]生物芯片北京国家工程研究中心,北京102206

出　　处：《清华大学学报（自然科学版）》2007年第9期1526-1530,共5页Journal of Tsinghua University(Science and Technology)

基　　金：国家"八六三"高技术项目(2006AA020701;2006AA020803);国家"九七三"基础研究基金项目(2006CB705700);浙江省科技攻关重点项目(2006C21G3210005);清华大学基础研究基金项目(052205003);清华-裕元医学科学研究基金项目(40000510B)

摘　　要：生物芯片提供一种重要的生物分子检测方法,在科研和医学应用领域被广泛关注。该文以大肠杆菌和黄单胞菌检测为例研究微生物的基因芯片鉴定方法和生物芯片的再生使用价值,采用一种自主研制的新型微阵列芯片扫描仪对微生物鉴定和生物芯片的重复利用开展理论与实验研究。结果表明,用基因芯片鉴定微生物是非常可靠的,所设计的生物分子探针与芯片表面修饰分子的结合很牢固,5次重复杂交清洗后依然可以产生良好的荧光信号,从而验证了再生芯片使用的可行性。为了提高生物芯片的重复使用次数,论文还对生物芯片的设计与荧光标记方法进行了分析讨论。Biochips provide important molecular biology detection methods for scientific research and for medical applications. This research investigates that bioehips can be reused. A new microbe identification method was developed using gene chips. Escherichia Coli and Xanthomonas Campestris were used as examples for the gene chips design to identify microbes with a microarray scanner built to test if the gene chip can be reused. Tests show that the gene chip can identify the microbes and that a tight joint is formed between the biological probe and the surface modification molecule. The fluorescence signal is still strong even after the gene chip has gone through five cycles of hybrid and washing, which shows the feasibility of reusing gene chips. Except for clinic applications which need to consider cross pollution, biochips can be reused to reduce costs. To further extend the biochip lifetime, this paper also analyzed the biochip design and fluorescent marker.

关 键 词：生物芯片 微阵列芯片扫描仪 荧光 再生芯片 

分 类 号：O43[机械工程—光学工程]