毛细管电泳电化学检测法测定中药枳实和枳壳中的辛弗林和3种黄酮  

Determination of Flavonoids in Fructus Anrantii Immaturus and Fructus Aurantii by Capillary Electrophoresis with Electrochemical Detection

作　　者：彭友元[1] 叶建农[2] 

机构地区：[1]泉州师范学院化学系,福建泉州362000 [2]华东师范大学化学系,上海200062

出　　处：《分析测试学报》2007年第5期694-697,共4页Journal of Instrumental Analysis

基　　金：福建省青年创新基金重点资助项目(2003J037)

摘　　要：采用毛细管电泳电化学检测法同时测定了枳实和枳壳中的辛弗林和3种黄酮即橙皮甙、柚皮素和柚皮甙的含量。考察了工作电极的氧化电位、分离电压和进样时间,运行缓冲的浓度和pH值对分离和检测的影响。在优化条件下,以300μm直径的碳圆盘电极为检测电极,检测电位为＋0.85 V（vs.SCE）,在80 mmol/L硼酸盐（pH 8.45）的运行缓冲液中,被测物浓度与峰电流在3个数量级范围内呈良好线性,检出限（S/N=3）在1×10^-4~5×10^-4g/L之间,该法简单、可靠、快速,单次测定可在20 m in内完成,已经成功应用于实际样品枳实和枳壳中4组分的测定,样品处理简单,无须预富集,检测结果令人满意。A simultaneous determination of synephrine,hesperidin,naringenin and naringin in Fructus anrantii Immaturus and Fructus aurantii by capillary electrophoresis with electrochemical detection（CE-ED） was reported.Effects of several important factors such as the pH and running buffer concentration,separation voltage,injection time,and detection potential were investigated to acquire the optimum conditions.Under the optimum conditions,the analytes could be separated within 20 min in a 75 cm length capillary at a separation voltage of 12 kV in a 80 mmol·L-1 borate buffer（pH 8.45）.A 300 μm diameter carbon disk electrode generates good response at ＋0.85 V（vs.SCE） for all analytes.The relationship between peak currents and analyte concentrations was linear over about three orders of magnitude with detection limits（S/N=3） ranging from 2×10-4-5×10-4 g·L-1 for all analytes.This proposed method demonstrated long-term stability and reproducibility with relative standard deviations of less than 4% for both migration time and peak current（n=7）.It has been successively used for the determination of these analytes in real samples,and the assay result was satisfactory.

关 键 词：毛细管电泳 电化学检测 枳实 枳壳 黄酮 辛弗林 橙皮甙 柚皮素 柚皮甙 

分 类 号：O657.8[理学—分析化学] Q949.752.7[理学—化学]