流动注射双安培法测定尿酸  

Flow Injection Biamperometric Analysis of Uric Acid

作　　者：张君才[1] 陈佑宁[1] 王亮亮[1] 

机构地区：[1]咸阳师范学院化学系,陕西咸阳712000

出　　处：《分析测试学报》2007年第5期718-721,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(50573046);陕西省教育厅科研基金资助项目(07JK424)

摘　　要：基于尿酸在预阳极化的铂电极上的催化氧化和不可逆电对的双安培检测原理,使用经过恒电位预阳极化处理的双铂电极,在外加电位差为0 V时,通过偶合尿酸在一支电极上的氧化和氧化铂在另一支电极上的还原两个不可逆电极过程,建立了一种快速准确在线分析测定尿酸的电化学新方法———流动注射双安培检测法。在外加电位差为0 V时尿酸的氧化电流与其浓度在1.0×10^-6～8.0×10^-3mol/L范围内呈线性关系（r=0.998 5,n=11）,检出限为5.0×10^-7mol/L。连续28次测定4.0×10^-4mol/L尿酸,电流值RSD为2.5%。电极的稳定性良好,常见无机离子和有机离子均不干扰测定。用该方法对尿酸含量进行了测定,样品处理简单,且有很高的选择性和灵敏度,结果令人满意。A simple,rapid,and accurate flow-injection electrochemical method for on-line determination of uric acid（UA） is established.The method is based on the electro-catalytic oxidation of UA at oxide-covered platinum electrode and the flow injection biamperometry use for irreversible couple system.Using two pre-anodized platinum electrodes with the applied potential difference of 0 V,the biamperometric detection system has been established by coupling the oxidation of UA and the reduction of platinum oxide.The linear relationship between the current and concentration of UA is obtained in the range of 1.0×10-6～8.0×10-3 mol/L（r=0.998 5,n=11） with the detection limit of 5.0×10-7 mol/L.The relative standard derivation of 2.5% was obtained for 28 successive determination of 4.0×10-4 mol/L UA.The method has been shown to be sensitive,selective,rapid and suitable for the on-line determination of uric acid.

关 键 词：尿酸 流动注射分析 双安培法 铂电极 不可逆电对 

分 类 号：O657.1[理学—分析化学] R696.6[理学—化学]