日本血吸虫可溶性虫卵抗原组分的分离与纯化  

The fractionation and purification of soluble egg antigen of Schistosoma japonicum

作　　者：金烈[1] 何立[1] 王薇[1] 张培喜[1] 宗红英[1] 

机构地区：[1]武汉大学基础医学院寄生虫教研室,430071

出　　处：《中国地方病学杂志》2007年第5期484-486,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金项目（30471509）

摘　　要：目的 分离和纯化日本血吸虫可溶性虫卵抗原（soluble egg antigens，SEA）各组分。方法 常规制备血吸虫可溶性虫卵抗原，经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（SDS—PAGE）柱电泳层析仪分离，分别按每管20、60、120min的流速各收集15管，并经透析纯化各抗原组分．再用SDS—PAGE检测并分析其相对分子质量（Mr）。结果 获得40种相对分子质量不同的可溶性虫卵抗原组分，时间流速对蛋白峰的出现和各组份中相对分子质量大小有明显的影响，当流速为20、60、120min，相对分子质量（×10^3）分别为34．19±4．56、60．35±11．10、91．08±12．75，组间比较差异有统计学意义（F=12、488，P〈0．05）。电泳图显示各蛋白峰均为清晰的单一带。结论 SDS—PAGE柱电泳层析方法能有效地分离出日本血吸虫可溶性虫卵抗原相对分子质量不同的抗原组分，流速明显影响分离结果。Objective To fractionate Schistosoma japonicum soluble egg antigen （SEA）. Methods SEA was prepared from the purified eggs of infected rabbit livers using conventional methods, which were fractionation by electrophoretic column chromatography and collection under a series of flowing speeds of 20,60,120 min per tube, followed by the analysis of relative molecular mass by SDS-PAGE test. Results Forty fractionated antigens were obtained, each fractionated antigens as tested by SDS-PAGE had a single protein band. Their relative molecular mass （Mr,× 10^3） were 34.19 ± 4.56, 60.35 ± 11.10 and 91.08 ± 12.75, respectively, and the differences between each group being significant （F = 12.488, P 〈 0.05）. Conclusions SDS-PAGE electrophoretic column chromatography effectively fractionate Schistosomajaponicum SEA into a series of antigens with different relative molecular mass and electrophoretic-purity degree. The electriphoretic flowing speeds affects the fractionated results apparently.

关 键 词：血吸虫 日本 虫卵可溶性抗原 电泳 聚丙烯酰胺凝胶 

分 类 号：R686[医药卫生—骨科学]