利用双向电泳技术分离盐胁迫下番茄叶片蛋白  

2D-PAGE Analysis of Wild Tomato Leaf Protein under Salt Stress

作　　者：白汝瑾[1] 庄天明[1] 刘杨[1] 杨冬冬[1] 陈火英[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101

出　　处：《上海交通大学学报（农业科学版）》2007年第4期363-366,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家"十五"863项目(No.2001AA67010);上海市科委重大专项(06DZ19132)

摘　　要：采用双向聚丙烯酰胺凝胶电泳技术,对耐盐性不同的野生番茄品种盐处理后进行蛋白质组分析。2种野生番茄(秘鲁番茄、潘那利番茄)双向电泳后,幼苗长至两叶一心时,用150 mmol·L-1 NaCl胁迫,14 d后用TCA丙酮法提取叶片总蛋白。经裂解、水化、等电聚焦、平衡、SDS-PAGE电泳和考马斯亮蓝胶体染色等一系列步骤后,番茄叶片总蛋白以蛋白点的形式呈现在凝胶图上。经ImageMaster 2D platinum软件分析,匹配率均达90%以上,图像分辨率也超过800个蛋白斑点。结合软件分析和肉眼观察,秘鲁番茄、潘那利番茄分别有16、15个蛋白被发现为上调蛋白,11、18个为下调蛋白。The protein expression patterns of leaves from two wild tomato species Lycopersicon peruvianum LA111 and Lycopersicon pennellii LA716 were compared when they grew under 150 mmol-L^-1 NaCl stress for 14 days. Tomato leaves were suspended in precooled TCA- acetone solution, dissolved in lysis buffer, and rehydrated in rehydration buffer. After IEF, equilibration and SDS-PAGE electrophoresis, the two dimensional electrophoresis maps were obtained. Captured by ImageScaner 5.0 and analyzed by ImageMaster 2D Platinum 5.0, each gel map showed more than 800 protein spots reproducibly identified. 16 protein spots were up-regulated and 11 were down-regulated in Lycopersicon peruvianum LAll 1; 15 were up-regulated and 18 were down-regulated in Lycopersicon pennellii LA716.

关 键 词：野生番茄 盐胁迫 双向电泳 

分 类 号：Q813.5[生物学—生物工程] TQ937[化学工程]