花生△^(12)-脂肪酸脱氢酶基因的克隆及序列分析  

Molecular Cloning and Sequence Analysis of △^(12)-fatty Acid Desaturase in Peanut(Arachis hypogaea L.)

作　　者：潘丽娟[1] 禹山林[1] 杨庆利[1] 闵平[1] 曹玉良[1] 

机构地区：[1]山东省花生研究所,山东青岛266100

出　　处：《花生学报》2007年第3期5-10,共6页Journal of Peanut Science

基　　金：国家"863"计划资助项目(2006AA10A114)

摘　　要：根据已报道的△12-脂肪酸脱氢酶基因(FAD)的氨基酸保守序列设计引物进行RT-PCR扩增,得到花生△12-脂肪酸脱氢酶基因881bp部分cDNA序列,然后通过快速扩增cDNA末端技术(RACE),向两端延伸得到1410bp的花生△12-脂肪酸脱氢酶基因全长cDNA序列。序列分析表明有一个长1140bp、编码379个氨基酸残基的开放阅读框,所编码蛋白质的大小约为43kDa。推测的氨基酸序列具有膜整合蛋白酶特异性的3个组氨酸保守区;氨基酸疏水性分析结果表明,所编码的氨基酸序列存在2个具有膜固定蛋白(membrance-anchored protein)重要特征的疏水结构,2个疏水区共跨膜4次,这些特性表明所获得的序列为△12-脂肪酸脱氢酶基因。To study the function of peanut △^12-fatty acid desaturase, the full-lengh cDNA of AhFAD was amplified by RACE from peanut seed. Sequence analysis found that the open reading frame encodes a protein with 379 amino acids and the molecular weight of deduced protein of AhFAD was 43 kDa. One highly conserved feature of all membrane-bound desaturases, was the presence of three histidine boxes, with the general sequence HXXXH. The hydrophobicity analysis showed that the sequence of the encoded amino acids had two hydrophobic structures sharing the characteristics of membrane-anchored protein, which totally crossed the membrane four times. These analyses revealed the obtained sequence was △^12-fatty acid desaturase.

关 键 词：花生 脂肪酸脱氢酶 基因克隆 序列分析 

分 类 号：S565.2[农业科学—作物学] Q78[生物学—分子生物学]