花生种子脂肪氧化酶缺失品种筛选研究  

Identification of Lipoxygenase Isozymes in Peanut Seeds

作　　者：陈静[1] 苗华荣[1] 石运庆[1] 吴兰荣 禹山林[1] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]青岛市种子站,山东青岛266000

出　　处：《花生学报》2007年第3期11-13,共3页Journal of Peanut Science

基　　金：山东省农科院青年科研基金项目(2005YQ005);山东省良种工程(高产优质出口专用花生新品种培育);国家科技支撑计划项目(高产优质特用花生育种技术研究及新品种选育)

摘　　要：根据花生种子脂肪氧化酶同工酶等电点差异,利用等电聚焦电泳技术,筛选花生种子脂肪氧化酶缺失的品种。结果表明:不同品种花生种子脂肪氧化酶的IEF-PAGE图谱有差异,有些花生品种呈现四条酶带,有些花生品种呈现三条酶带,缺失一条酶带。Based on difference of peanut lipoxygenase pI, a method was developed to identify peanut lipoxygenase by IEF-PAGE. The isoenzymes of lipoxygenase from different varieties of peanut were identified by the method. The results showed ： There were 4 bands of lipoxygeanse in varieties including Qixiakangpisanlihong , Tanchengshuiguozi and A. SP, but there were 3 bands of lipoxygenase in others.

关 键 词：花生 种子 脂肪氧化酶 等电聚焦电泳 

分 类 号：S565.2[农业科学—作物学] Q946[生物学—植物学]