利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系  

Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers

作　　者：纪剑辉[1] 曹爱忠[1] 王海燕[1] 覃碧[1] 王苏玲[1] 孔芳[1] 陈佩度[1] 刘大钧[1] 王秀娥[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室细胞遗传所,南京210095

出　　处：《遗传》2007年第10期1256-1262,共7页Hereditas（Beijing)

基　　金：高校博士点基金课题(编号:20020307011);国家高技术研究发展计划(863计划)(编号:2006AA10Z1F6);长江学者和创新团队发展计划;教育部科学技术重大研究项目(编号:10418)资助~~

摘　　要：采用定位于小麦2B染色体上的72对分子标记对含小麦抗白粉病基因Pm6的8份普通小麦(T.aestivum L.)-提莫菲维(T.timopheevii zhuk.)渐渗系材料进行分析,通过分子标记标图确定8份材料中渗入的提莫菲维小麦染色体片段的大小,同时结合连锁图谱对这些材料进行了遗传和物理标图。参考本研究所用的分子标记在染色体2B上的定位结果,Pm6基因被位于2B染色体长臂近末端2BL-6区域,提莫菲维小麦2G染色体渐渗片断长度由短到长排列顺序为:IGV1-465<IGV1-464<IGV1-463<IGV1-468<(IGV1-448、IGV1-458)<IGV1-474<IGV1-466。研究结果与陶文静(1999)采用RFLP标记标图的结果基本吻合,进一步确认IGV1-465渐渗片段最小。本研究所鉴定的基于PCR的分子标记还可以用于区分该套渐渗系,这为这套材料在育种中的分子标记辅助选择和Pm6基因的克隆奠定了基础。In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers （including SSR and STS primers） were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the analyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the region of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow （from short to long）： IGV1-465〈IGV1-464〈IGV1-463〈IGV1-468〈（IGV1-448,IGV1-458）〈IGV1-474〈 IGV1-466. The results were coincide with the RFLP results and further confirmed that line IGV1-465 contained the minimum introgression segment. Using the 10 polymorphism PCR markers identified in the present research, it is feasible to differentiate seven introgression lines. This information will be helpful for marker assistant identification of these lines in wheat breeding program and for further isolation of the Pm6 gene.

关 键 词：小麦 提莫菲维小麦 白粉病 Pm6 渐渗系 

分 类 号：S512.1[农业科学—作物学]