毒蕈乙酰胆碱受体亚型关系的研究  

Relationship between muscarinic acetylcholine receptor subtypes

作　　者：于海龙[1] 肖云[1] 艾静 李霞[1] 宫滨生[1] 

机构地区：[1]哈尔滨医科大学生物信息学系,哈尔滨150086 [2]黑龙江省生物医药重点实验室,哈尔滨150086

出　　处：《遗传》2007年第10期1280-1288,共9页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30170515;30571034;30570424;30600367);国家高技术研究发展计划项目(863计划)(编号:2003AA2Z2051);黑龙江科技攻关(编号:GB03C602-4);黑龙江自然科学基金(编号:F2004-02);哈尔滨医科大学青年科学基金(编号:060045)项目资助~~

摘　　要：利用不同类型的生物学数据,运用生物信息学方法和策略,从分子进化、序列相似性、表达相关性以及蛋白互作4个层面对M受体亚型之间的关系进行了比较全面的研究。分析表明,从分子进化和序列相似性角度,毒蕈乙酰胆碱受体5种亚型可分为2个亚类,分别为M1、M3、M5亚类(第一亚类)与M2、M4亚类(第二亚类),每一亚类内部亚型之间进化距离相对较近,序列相似性较高。在表达层面发现第一亚类中受体亚型与第二亚类中受体亚型在某些组织中正表达相关,呈现共表达趋势。在互作层面发现两亚类之间受体亚型存在着间接互作的关系,呈现协同作用的现象。Muscarinic acetylcholine receptor belongs to the G-coupled receptor family, the cooperation between its five subtypes is crucially important in maintaining the normal physiological function. At present, many types of biological resources are increasing, which provides unprecedented opportunities to study the relationships between muscarinic acetylcholine receptor subtypes. In this study, we take advantage of different types of data, using bioinformatics tools and strategies, to analyze the relationship between muscarinic acetylcholine receptor subtypes from four aspects, including evolution, sequence similarity, expression correlation and protein-protein interaction network. From evolution and sequence similarity aspects, we found the five subtypes of muscarinic acetylcholine receptor can be classified into two subclasses. The first subclass includes M1, M3, and MS, and the second subclass includes M2 and M4. The evolutionary distance between two subtypes of the same subclass is relatively near, and the sequence similarity between them is relatively high. From expression correlation aspect, we found that the subtypes of the first subclass have a positive correlation with the subtypes of the second subclass, that is, there are potential co-expression relationships between them. From protein-protein interaction aspect, we found that the subtypes of the first subclass have indirect interactions with the subtypes of the second subclass, which indicates cooperation relationships.

关 键 词：毒蕈乙酰胆碱受体 亚型 分子进化 表达相关性 蛋白质互作 

分 类 号：Q51[生物学—生物化学]