重楼含药血清对大鼠系膜细胞增殖及凋亡的影响  

Effects of Rhizoma paridis pharmacologic serum on rat mesangial cell proliferation and apoptosis in vitro

作　　者：黄谷香[1] 刘瑞洪[1] 

机构地区：[1]中南大学湘雅二医院老年科,长沙410011

出　　处：《中华肾脏病杂志》2007年第9期589-592,共4页Chinese Journal of Nephrology

摘　　要：目的探讨重楼治疗肾小球疾病的作用机制。方法以脂多糖诱导MC增殖。提取大鼠的含药血清作用于体外培养的大鼠系膜细胞（MC），观察重楼对MC增殖、凋亡的影响。用活细胞计数（CCK．8）法检测细胞增殖。以Hoechst染色及流式细胞仪检测细胞凋亡。用RT-PCR检测bcl-2mRNA水平。结果重楼含药血清可抑制MC异常增殖、诱导MC凋亡，呈剂量依赖性。重楼各剂量组MC凋亡率[低、中、高剂量组分别为（11．72±0．32）％、（19．76±0．35）％、（18．71±0．41）％1较脂多糖组[（4．68±0．25）％】均显著增高（P均〈0．01），重楼中剂量组与低剂量组间差异有统计学意义（P〈0．01）。重楼各剂量组MC bcl-2mRNA表达[低、中、高剂量组分别为（51．06±0．77）％、（44．06±0．66）％、（35．68±0．67）％】较脂多糖组[（59．62±1．12）％】显著减少（P〈0．01）。重楼呈剂量依赖性降低MCbcl-2mRNA表达水平（P〈0．01）。结论重楼含药血清可抑制MC增生和诱导MC凋亡，其机制可能与抑制抗凋亡基因bcl-2mRNA的表达有关。Objective To investigate the effects of Rhizoma paridis pharmacologic serum on rat mesangial cell （MC） proliferation and apoptosis, and to explore the underlying mechanism. Methods Sera containing Rhizoma paridis （low, moderate and high dosages） and the positive control medicine such as muhiglycosides Trioterygil wilfordii （MTW） and prednisone were prepared and put together with lipopolysaccharide （LPS）. Rat MC was cultured in vitro for 12 h, 24 h and 36 h respectively. The effects of the drugs-containing sera on the proliferation of MC were observed by Cell Counting Kit-8 （CCK-8） colorimetric assay. Apoptosis and apoptosis rate of MC were detected respectively by Hoechst 33258 fluorescence staining method and flow cytometry. The expression of bcl-2 mRNA of MC was evaluated by reverse transcription polymerase chain reaction （RT-PCR） method. Results Compared with LPS group, A amounts of Rhizoma paridis groups, MTW group and prednisone group were significantly decreased（P〈0.01 or P〈0.05）. Rhizoma paridis pharmacologic serum inhibited MC proliferation in a dose-dependent and time-dependent manner. MC apoptosis was obviously induced by Rhizoma paridis pharmacologic serum, in a dose-dependent manner. Apoptosis rates of MTW group were significantly higher than those of all Rhizoma paridis groups. Prednisone pharmacologic serum did not induce MC apoptosis. Rhizoma paridis pharmacologic serum inhibited expression of MC bcl-2 mRNA （P〈0.01）. Compared with MTW, the inhibitory effect of Rhizoma paridis was less significant. Prednisone pharmacologic serum did not inhibit expression of MC bcl-2 mRNA. Conclusions Rhizoma paridis pharmacologic serum can partially inhibit LPS-induced MC proliferation and induce MC apoptosis. Rhizoma paridis pharmacologic serum can down-regulate the expression of bcl-2 mRNA, which partly explains the mechanism of Rhizoma paridis pharmacologic serum induced apoptosis. Rhizoma paridis can improve proliferative glomerulopathy possibly through inhibiting prolifera

关 键 词：重楼 凋亡 BCL-2 系膜细胞 

分 类 号：R686[医药卫生—骨科学]