脂欣康胶囊对血管平滑肌细胞源性泡沫细胞CD36表达的影响  

The Effect of Zhixinkang Capsule on the Expression of CD36 in Foam Cell Originated From Vascular Smooth Muscle Cell in Rats

作　　者：朱莹[1] 张文高[1] 郑广娟[1] 姜浩[1] 

机构地区：[1]山东中医药大学,山东省济南市250014

出　　处：《中国动脉硬化杂志》2007年第7期481-483,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30472275)

摘　　要：目的探讨脂欣康胶囊对动脉粥样硬化泡沫化细胞形成的可能调控机制,观察脂欣康胶囊及洛伐他汀干预大鼠血管平滑肌细胞源性泡沫细胞CD36mRNA表达的变化。方法用组织贴块培养法培养血管平滑肌细胞,以氧化型低密度脂蛋白使其泡沫化,并以脂欣康胶囊和洛伐他汀分别干预。采用流式细胞仪和原位杂交技术观察CD36mRNA表达的变化。结果与生理盐水组和空白对照组相比,脂欣康胶囊与洛伐他汀均能降低CD36mRNA的表达(P<0.01),且脂欣康组较洛伐他汀组下降更显著(P<0.05)。结论脂欣康与洛伐他汀均能抑制CD36的表达,并且其作用优于洛伐他汀。其作用机制可能为脂欣康的益气活血、解毒化浊之功使平滑肌细胞内蕴藏的痰浊瘀毒得以疏布排泄于外。这可能是其抑制血管平滑肌细胞增殖和泡沫化细胞形成的机制之一。Aim To approach Zhixinkang possible regulation mechanism for atherosclerosis （As） foam cell, and to investigate the effect of Zhixinkang and Lovastatin on CD36 mRNA in foam cell originated from vascular smooth muscle cell （ VSMC）. Methods The cultured VSMC were loaded with oxidize low density lipoprotein （ ox-LDL） and interfered in Zhixinkang and Lovastatin. The expression of CD36 mRNA were detected with flow cytometer and In Situ hybridization and analyzed with statistics. Results Zhixinkang and Lovastatin could decrease CD36 mRNA expression than that in the isotonic Na chloride group and the blank group. Moreover, CD36 rnRNA expression in Zhixinkang group was different from that in Lovastatin group. Conclusion Zhixinkang could decrease CD36 rnRNA expression, which was superior to Lovastatin. Zhixinkang had the effect of tonifying qi and pro＇noting blood, neutralizing poison and resolving turbid in VSMC, which was one of mechanism to inhibit the multiplication of VSMC and restrain the formation of foam cell.

关 键 词：内科学 脂欣康胶囊 血管平滑肌细胞源性泡沫细胞 CD36 流式细胞仪 原位杂交 

分 类 号：R5[医药卫生—内科学]