金钱草片的薄层鉴别及有效成分含量测定  

Identification and Quantitative Determination of Jinqiancao Tablet

作　　者：高向军[1] 包小红[1] 周娟[1] 

机构地区：[1]四川省食品药品检验所,四川成都610036

出　　处：《中国现代中药》2007年第9期16-17,20,共3页Modern Chinese Medicine

摘　　要：目的:建立金钱草片的鉴别和含量测定方法。方法:采用TLC鉴别金钱草片中的金钱草药材,HPLC测定金钱草片中槲皮素和山柰素的含量。结果:槲皮素在0.0027～0.1075μg线性关系良好(r=0.9999);山柰素在0.0164～0.4912μg线性关系良好(r=0.9999)。槲皮素平均回收率为101.13%,RSD=2.40%;山柰素平均回收率为98.66%,RSD=1.22%。结论:鉴别重复性好,专属性强,含量测定方法简便、准确。Objective： To establish identification and quantitative determination method of Jinqiancao tablet. Methods： The TLC and HPLC were used. Results： The TLC methods could identify Herba Lysimachiae. The HPLC method could quantitatively determine quercetin and kaempferol content in Jinqiancao tablet. The linear range of quercetin was0. 002 7 -0. 107 5μg （r =0. 999 9） , the average recovery was 101.13%with RSD of 2.40%. The linear range of kaempferol was 0. 016 4 -0. 491 2μg （r = 0. 999 9）, the average recovery was 98.66% with RSD of 1.22%. Conclusion： Identification methods are reproducible and specific, quantitative determination method is simple and accurate.

关 键 词：金钱草片 薄层色谱法 高效液相色谱法 槲皮素 山柰素 

分 类 号：R69[医药卫生—泌尿科学]