红千层愈伤组织诱导及植株再生  

Callus Induction and Plant Regeneration in Callistemon rigidus

作　　者：伍成厚[1] 冯毅敏[1] 叶振华[1] 朱纯[1] 龙丽萍[1] 

机构地区：[1]广州市园林科学研究所

出　　处：《经济林研究》2007年第3期11-14,共4页Non-wood Forest Research

基　　金：广东省科技计划项目(2004B60302006);广州市科技计划项目(004Z3-E0361)

摘　　要：以茎段、芽和叶片为材料,研究了红千层愈伤组织诱导及植株再生的方法。结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养愈伤组织可发育成绿色和粉红色2种类型,其中绿色、致密的愈伤组织可以分化出不定芽。培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%。Taken stem segment, bud and leaf as materials, the means of callus induction and plant regeneration in Callistemon rigidus R. Br. were studied. The results showed that callus could be induced from all types of the materials, pink and green callus could be gain through subculture, and adventitious buds could be induced from the compacted greens. The optimal medium for inducing adventitious bud was 1/2MS supplemented with 6-BA1. 0 mg/L and NAA0. 1 mg/L, and the optimal one for test-tube plantlets rooting was 1/2MS supplemented with IBA0. 25 mg/L, in which the rooting rate could be up to 95 %.

关 键 词：红千层 愈伤组织诱导 植株再生 组织培养 

分 类 号：S685.99[农业科学—观赏园艺] Q945.52[农业科学—园艺学]