微生物修复氯丁唑污染土壤的研究  

Microbial Remediation Technology of Soil Contaminated by Paclobutrazol

作　　者：王锐刚[1] 张传义[1] 张雁秋[1] 

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221008

出　　处：《农业环境科学学报》2007年第5期1667-1671,共5页Journal of Agro-Environment Science

摘　　要：氯丁唑是一种植物生长调节剂,难溶于水,在土壤中残留时间较长。本文通过循环富集筛得氯丁唑高效降解菌,研究了环境条件对其降解氯丁唑效果的影响,进行了受氯丁唑污染土壤修复的研究,为微生物法修复氯丁唑污染土壤提供依据。结果表明,筛选出的菌种是假单胞杆菌和芽孢杆菌;在液体有降解菌培养基中振荡40d氯丁唑降解率达98%,降解菌能降解氯丁唑产生CO2;通气量、培养基、温度、光照、含水量等都会对降解氯丁唑效果产生影响,微生物降解氯丁唑需要大量的O2,土壤中富含有机质时会影响微生物对氯丁唑的降解,在降解菌生长温度范围内,提高温度有利于对氯丁唑的降解,光照也有利于氯丁唑的分解等。将降解菌投放于受氯丁唑污染土壤,通过光照、通气等,40d后土壤中氯丁唑的降解率达85%,土壤中微生物含量恢复到正常值的90%。Paclobutrazol, insoluble in water and long rettmant time in soil, is a sort of plant growth regulator. The high efficiency degrading bacteria were obtained by circulation culture and the conditions influencing degradation efficiency of paclobutrazol were studied to provide basis for microbial remediation of the soil contaminated by paclobutrazol. The high efficiency degrading bacteria being obtained were pseudomonas and bacillus, 98% of paclobutrazol could be degraded by the degrading bacteria after 40 d under vibration condition in liquid culture medium. Paclobutrazol was degraded to CO2 by mixed degrading bacteria. Aeration, culture medium, temperature, illumination and water content can influence degradation efficiency of paclobutrazol. Plenty of O2 needed to be provided for the degrading bacteria to degrade paclobutrazol. Organic matter in soil also influenced the degradation efficiency of paclobutrazol. High temperature and illumination was in favor of paclobutrazol degradation by the degrading bacteria. When the degrading bacteria inoculated into the soil contaminated by paclobutrazol, plenty of oxygen and light were adsorbed by soil, about 85% of paclobutrazol was degraded and the microbe biomass reached 90% of the normal level in soil after 40 d.

关 键 词：微生物修复 氯丁唑 土壤 

分 类 号：X592[环境科学与工程—环境工程] X172