地尔硫对缺氧所致血管内皮细胞线粒体膜电位变化的保护作用  

Effects of Diltiazem on Intracellular Mitochondria Membrance Potential of Human Vascular Endothelial Cells under Hypoxia

作　　者：马贵喜[1] 李鸣皋[1] 韩磊[1] 蒙果[1] 刘玉[1] 李靖[1] 黄友章[2] 

机构地区：[1]海军总医院航空潜水医学中心,北京100037 [2]海军总医院血液科,北京100037

出　　处：《海军总医院学报》2007年第3期129-131,共3页Journal of Naval General Hospital of PLA

基　　金：海军后勤部科研基金(01-3310)

摘　　要：目的观察地尔硫对人大血管内皮细胞缺氧损伤时线粒体膜电位的影响。方法将培养的血管内皮细胞分为对照组、单纯缺氧组和加入地尔硫卓后再缺氧组,应用激光共聚焦显微镜扫描,并测定标记的血管内皮细胞每秒钟线粒体膜电位值,观察缺氧前后人大动脉血管内皮细胞线粒体膜电位变化。结果与对照组相比,单纯缺氧组的线粒体膜电位水平显著降低(P<0.01);加入地尔硫后再进行缺氧组与单纯缺氧组相比,内皮细胞线粒体膜电位水平有显著升高(P<0.01),与对照组相比内皮细胞线粒体膜电位水平变化不明显(P>0.05)。结论缺氧引起血管内皮细胞线粒体膜电位降低;地尔硫艹卓可抑制缺氧损伤所致的线粒体膜电位的降低,从而具有稳定线粒体膜电位、保护血管内皮细胞的作用。Objective To study the effects of hypoxia on intracellular mitochondria membrane potential（MMP） of human vascular endothelial cells and the protection of the Diltiaze. Methods The changes of intracellular MMP were detected, using laser scanning confocal microscope, in vascular endothelial cells divided into control group, hypoxia group and hypoxia plue Diltiazem group. Results Compared with control group,the intracellular MMP of pure hypoxia group significantly decreased （P〈0.01）. Addition of Diltiazemcom in pure hypoxia group significantly increased the intracellular MMP （P 〈 0.01）, which was not significantly different compared with control group （P〉0.05）. Conclusion Hypoxia has significant influences on the intracellular MMP, Diltiazem has significant protection to intracellular MMP decrease resulted from hypoxia.

关 键 词：地尔硫(艹卓) 线粒体膜电位 缺氧 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R972.3[医药卫生—基础医学]