低剂量安体舒通对兔肥厚心室肌电重构的影响  

Effect of electrical remodeling in hypertrophied myocardium caused by low dose aldactone alone

作　　者：于厚志[1] 杨庆[1] 邓珏琳[1] 陈茂[1] 黄德嘉[1] 

机构地区：[1]四川大学华西医院

出　　处：《山东医药》2007年第23期13-15,共3页Shandong Medical Journal

基　　金：国家自然科学基金(39970303)。

摘　　要：目的研究低剂量安体舒通对肥厚心室肌电生理特性的影响。方法将30只纯种日本大耳白兔随机分为安体舒通组、环缩组和对照组各10只,安体舒通组和环缩组环缩腹主动脉构建左心室肥厚模型,对照组仅游离腹主动脉(不结扎)。术后第2天安体舒通组喂服安体舒通10mg/kg,对照组和环缩组喂服安慰剂;连续8周后停药1d。用心脏电刺激单相动作电位记录技术测定心室MAP时程(MAPD_(90))和心室有效不应期(VERP),用免疫印迹方法测定反向钠-钙交换(NCX)蛋白表达。结果环缩组和安体舒通组心脏重量/体质量明显高于对照组,快速刺激后VERP明显长于对照组,NCX表达水平均显著高于对照组,P均<0.05;VERP延长时间(ΔVERP)与NCX表达水平明显相关(r=0.68)。结论单用低剂量安体舒通不能明显延缓心肌肥厚的发展,对肥厚心室肌频率相关性电重构亦无明显影响;此可能与其不能下调肥厚心室肌NCX高表达有关。[ Objective] To determine whether aldactone alone can have an impact on the electrical remodeling in the hypertrophied myocardium. Methods Rabbits were randomized as three group： constricted group（ n = 8） , control group （n = 7 ） and constricted ＋ aldactone group（ n = 6）. Left ventricular hypertrophy was induced in rabbits by abdominal aortic constriction, Sham-operated control rabbits underwent the same operation, except that the arota was not constricted. One day after surgery, the constricted and sham-operated animals were receive vehicle for a total duration of 8 weeks, while the constricted ＋ aldactone rabbits were given aldactone （ 10 mg/kg） for the same duration. Eight weeks after feeding, the electrophysiological parameters were recorded by the monophasic action potential recording technique in three sites including right ventricular outflow tract （RVOT） , left ventricular outflow tract （LVOT） and left ventricular apex （LVA）. The myocardial Na^＋ -Ca^2＋ exchanger protein was semiquantitated by western-blot analysis. Results Compared with the sham-operated control rabbits, the heart-to-bedy weight ratio was increased significantly in the constricted rabbits and constricted ＋ aldactone rabbits, 2.9 ±0. 31 versus 2.20 ±0.05 （ P 〈 0. 05） and 2.83 ± 0.32 versus 2.20 ± 0.05 （ P 〈 0. 05 ）, the latter two groups were not different significantly. Compared with the control rabbits, 90% of monophasic action potential duration after short-time rapid right atrial stimulation in the three sites （RVOT, LVOT and LVA） was not different significantly in the constricted rabbits and constricted ＋ aldactone rabbits, effective refractory period after rapid stimulation increased significantly in the left ventricular site of the latter two groups, （ 18±6）ms versus （8±4）ms and （ 16±8）ms versus （8±4）ms in the LVOT site, （20±6）ms versus （8±5）ms and（ 17±7）ms versus （8±5）ms,but the increase was not different significantly in the latte

关 键 词：左心室肥厚 有效不应期 单相动作电位 钠钙交换体 安体舒通 

分 类 号：R337.5[医药卫生—人体生理学]