高效阴离子交换色谱分离-脉冲安培检测法测定烟草料液中的糖、糖醇和醇类化合物  

Determination of Sugars and Sugar Alcohols in Tobacco Feed Liquids by High Performance Anion-Exchange and Pulsed Amperometric Detection

作　　者：唐坤甜[1] 梁立娜[1] 蔡亚岐[1] 牟世芬[1] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《分析化学》2007年第9期1274-1278,共5页Chinese Journal of Analytical Chemistry

基　　金：国家重点基础研究计划(973项目)(No.2003CB415001);中国科学院重要方向性项目(No.KZCX3-SW-432)

摘　　要：建立了高效阴离子交换色谱分离-脉冲安培检测法测定烟草料液中的糖、糖醇以及醇类化合物的方法。以NaOH为淋洗液，在CarboPac MA1阴离子交换柱上等度分离了肌醇、甘油、丙二醇、半乳糖醇、木糖醇、山梨醇、甘露醇、葡萄糖、果糖、蔗糖、甘露糖和半乳糖等12种化合物。对影响分离和检测的条件进行了优化，并在此优化条件下分析了烟草料液中的糖、糖醇以及醇类化合物。12种化合物的检出限为2．0～216μg／L（25．0μL进样，以3倍信噪比计算检出限）。12种化合物浓度为1～5mg／L的标准溶液连续7次进样的RSD为0．7％～4．3％。方法对烟草料液中12种化合物的加标回收率为80％～108％。方法灵敏、高效、简便、快捷。A chromatographic method was developed for the determination of inositol, galactitol, glycerol, xylitol, propylene glycol, sorbitol, mannitol, glucose, fructose, sucrose, mannose and galactose in tobacco feed liquid samples using high-performance anion-exchange chromatography coupled with pulsed amperometric detection. The separation was carried out on CarboPac MA1 anion-exchange column using NaOH eluent. Moreover, the conditions affecting the separation and detection were optimized. The detection limits ranged from 2.0 to 7.6 μg/L and the linear range of 0.1 to 50 mg/L was obtained for inositol, galactitol, glycerol, xylitol. The detection limits ranged from 9.0 to 11.9 μg/L and the linear range of 1 to 50 mg/L was obtained for propylene glycol, sorbitol, mannitol, glucose, mannose and galactose. For fructose and sucrose, the detection limits were 102 μg/L, 216μg/L respectively and linear range was 2 -100 mg/L （25.0 μL injection, S/N = 3 ）. The RSD of the twelve analytes （1.0 -5.0 mg/L） was 0.7% -4.3% （n =7）. In addition, the spiked recoveries of the twelve analytes in tobacco feed liquid were 80% - 108%.

关 键 词：高效阴离子交换色谱 脉冲安培检测 糖醇 糖 烟草料液 

分 类 号：TS47[农业科学—烟草工业]