N-(4-甲基苯甲酰基)苯甘氨酸手性固定相的合成及对多种手性化合物的拆分  

Preparation of N-(4-Methylbenzoyl)phenylglycine Stationary Phase and Its Application to the Separation of Various Enantiomers

作　　者：周玲玲[1] 李国祥[1] 王剑瑜[1] 袁黎明[1] 

机构地区：[1]云南师范大学化学化工学院,昆明650092

出　　处：《分析化学》2007年第9期1301-1304,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30160092);高等学校青年教学科研奖励计划(No.20011298);云南省自然科学基金(No.2005E0006Z)

摘　　要：合成了N-(4-甲基苯甲酰基)苯甘氨酸手性固定相,采用湿法装柱(250mm×2.0mm i.d.),在V(正己烷)∶V(异丙醇)=90∶10的高效液相色谱正相色谱条件下,测得理论塔板数为40800m-1,该柱成功地分离了醇类、胺类、氨基酸的对映异构体以及一些手性药物。结果表明:所拆分的16种手性化合物,有12种手性化合物得到基线分离,最好的分离度Rs=4.66,表现出良好的手性分离性能。N-（4-methylbenzoyl） phenylglycine chiral stationary phase was synthesized with 4-methylbenzoyl chlor/de, 4-methylbenzoyl phenylglycine and 3-aminopropyl silica gel, and its chiral column （250 mm × 2.0 mm, i.d. ） was prepared. Under the nornal high performance liquid chromatographic conditions such as hexane/isopropanol （90： 10, V/V） mobile phase with a flow rate of 0.3 mm/min and 254 nm of UV detection wavelength, the column performance is up to 40800 plates/m. Twelev of the sixteen enantiomers enantiomers including alcohols, amines, amino acids and chiral drugs, could be baseline separated and the best value of resolution factors （Rs） was 4.66. The results show that the chiral column possesses a good separation ability to chiral compound.

关 键 词：N-(4-甲基苯甲酰基)苯甘氨酸 手性固定相 高效液相色谱 手性拆分 

分 类 号：O657.7[理学—分析化学]