PCB_(153)诱导小鼠胚胎细胞凋亡对胚胎生长发育的影响  

Effect of PCB_(153) induced-apoptosis on mouse embryonic development

作　　者：常青[1] 赵泽文[1] 李泽桂[2] 梁志清[1] 

机构地区：[1]第三军医大学西南医院妇产科,重庆400038 [2]第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2007年第19期1838-1840,共3页Journal of Third Military Medical University

基　　金：重庆市科技计划项目(2002-10-77)~~

摘　　要：目的探讨环境雌激素多氯联苯同系物PCB153对发育过程中小鼠胚胎细胞凋亡的影响。方法于体外施予孕8.5d小鼠胚胎不同浓度PCB153,应用植入后全胚胎培养技术培养胚胎;采用原位TUNEL技术观察胚胎细胞凋亡。结果PCB153对胚胎发育具有明显影响且呈剂量-效应关系,且随PCB153浓度增加,凋亡细胞比值上升。结论PCB153能诱导胚胎细胞凋亡,这与其致胚胎发育异常有关,可能是PCB153的致畸作用机制之一。Objective To explore the effects of polychlorinated biphenyl congener （ PCB153 ） an environmental estrogen, on embryonic development and its mechanism. Methods Mouse embryos of gestational day 8.5 were exposed to different doses of PCB153 ex vitro. Post-implantation whole embryo culture （WEC） technique was used to investigate the effects of PCB153 on growth and development. The terminal deoxynucleotidyl transferase （TdT）-mediated dUTP nick-end labelling （TUNEL） technique was used to detect apoptosis in embryonic tissue sections. Results PCB153 had obvious effects on the development and growth of embryos in a dose-dependent manner; The ratio of apoptotic cells was increased with increased doses of PCB153. Conclusion PCB153-induced apoptosis is involved in mouse embryo anomalies.

关 键 词：植入后全胚胎培养 PCB153 胚胎发育 细胞凋亡 致畸机制 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]